Anti-p60 katanin antibody (ab67206)
Key features and details
- Mouse polyclonal to p60 katanin
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-p60 katanin antibody
See all p60 katanin primary antibodies -
Description
Mouse polyclonal to p60 katanin -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein within Human p60 katanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- p60 katanin transfected 293T cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab67206 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent dilution. Detects a band of approximately 34 kDa (predicted molecular weight: 56 kDa).
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Notes |
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WB
Use at an assay dependent dilution. Detects a band of approximately 34 kDa (predicted molecular weight: 56 kDa). |
Target
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Function
Severs microtubules in vitro in an ATP-dependent manner. This activity may promote rapid reorganization of cellular microtubule arrays, such as during disassembly of interphase microtubules at the G2-M transition. May also be required for microtubule release from the centrosome after nucleation. In mitotic spindles this could allow depolymerization of the microtubule end proximal to the centrosome, and subsequent poleward microtubule flux. In neurons, microtubule release within the cell body may allow their subsequent transport into neuronal processes by microtubule dependent motor proteins. This transport is required for axonal growth. -
Sequence similarities
Belongs to the AAA ATPase family. -
Domain
The N-terminus is sufficient for interaction with microtubules, although high affinity binding requires an intact C-terminal domain and ATP, which promotes oligomerization. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Predominantly cytoplasmic. Also localized to the interphase centrosome. Enhanced recruitment to the mitotic spindle poles requires microtubules and interaction with KATNB1. - Information by UniProt
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Database links
- Entrez Gene: 11104 Human
- Entrez Gene: 23924 Mouse
- Omim: 606696 Human
- SwissProt: O75449 Human
- SwissProt: Q9WV86 Mouse
- Unigene: 450175 Human
- Unigene: 28127 Mouse
- Unigene: 421297 Mouse
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Alternative names
- Katanin p60 ATPase containing subunit A1 antibody
- Katanin p60 ATPase-containing subunit A1 antibody
- Katanin p60 subunit A1 antibody
see all
Images
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All lanes : Anti-p60 katanin antibody (ab67206)
Lane 1 : p60 katanin transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Predicted band size: 56 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted? -
p60 katanin expression in NIH/3T3.
Datasheets and documents
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Datasheet download
References (1)
ab67206 has been referenced in 1 publication.
- Butler R et al. Genetic and chemical modulation of spastin-dependent axon outgrowth in zebrafish embryos indicates a role for impaired microtubule dynamics in hereditary spastic paraplegia. Dis Model Mech 3:743-51 (2010). WB ; Zebrafish . PubMed: 20829563