Recombinant Anti-p60 katanin antibody [EPR5071] (ab111881)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5071] to p60 katanin
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-p60 katanin antibody [EPR5071]
See all p60 katanin primary antibodies -
Description
Rabbit monoclonal [EPR5071] to p60 katanin -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa and HepG2 cell lysates. ICC/IF: HeLa cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5071 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab111881 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
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ICC/IF |
1/100.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 56 kDa. |
ICC/IF
1/100. |
Target
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Function
Severs microtubules in vitro in an ATP-dependent manner. This activity may promote rapid reorganization of cellular microtubule arrays, such as during disassembly of interphase microtubules at the G2-M transition. May also be required for microtubule release from the centrosome after nucleation. In mitotic spindles this could allow depolymerization of the microtubule end proximal to the centrosome, and subsequent poleward microtubule flux. In neurons, microtubule release within the cell body may allow their subsequent transport into neuronal processes by microtubule dependent motor proteins. This transport is required for axonal growth. -
Sequence similarities
Belongs to the AAA ATPase family. -
Domain
The N-terminus is sufficient for interaction with microtubules, although high affinity binding requires an intact C-terminal domain and ATP, which promotes oligomerization. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Predominantly cytoplasmic. Also localized to the interphase centrosome. Enhanced recruitment to the mitotic spindle poles requires microtubules and interaction with KATNB1. - Information by UniProt
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Database links
- Entrez Gene: 11104 Human
- Omim: 606696 Human
- SwissProt: O75449 Human
- Unigene: 450175 Human
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Alternative names
- Katanin p60 ATPase containing subunit A1 antibody
- Katanin p60 ATPase-containing subunit A1 antibody
- Katanin p60 subunit A1 antibody
see all
Images
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All lanes : Anti-p60 katanin antibody [EPR5071] (ab111881) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 56 kDa -
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling p60 katanin with ab111881 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing staining on the centrosome of Hela cells.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab111881 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab111881 has been referenced in 4 publications.
- Wenzel DM et al. Comprehensive analysis of the human ESCRT-III-MIT domain interactome reveals new cofactors for cytokinetic abscission. Elife 11:N/A (2022). PubMed: 36107470
- Leung JC & Cassimeris L Reorganization of paclitaxel-stabilized microtubule arrays at mitotic entry: roles of depolymerizing kinesins and severing proteins. Cancer Biol Ther 20:1337-1347 (2019). PubMed: 31345098
- Mirvis M et al. Primary cilium loss in mammalian cells occurs predominantly by whole-cilium shedding. PLoS Biol 17:e3000381 (2019). PubMed: 31314751
- Fu W et al. The role of katanin p60 in breast cancer bone metastasis. Oncol Lett 15:4963-4969 (2018). PubMed: 29552132