Overview

  • Product nameAnti-Kazrin antibody
    See all Kazrin primary antibodies
  • Description
    Mouse polyclonal to Kazrin
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-415 of Human Kazrin (NP_001018000.1)

  • Positive control
    • Rat brain tissue lysate and transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab88752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 86 kDa.

Target

  • FunctionComponent of the cornified envelope of keratinocytes. May be involved in the interplay between adherens junctions and desmosomes. The function in the nucleus is not known.
  • Tissue specificityIsoform 2, isoform 3 and isoform 4 are expressed in several cell lines including keratinocytes and bladder and epidermoid carcinoma (at protein level). Isoform 2, isoform 3 and isoform 4 are expressed in hair follicle and interfollicular epidermis (at protein level).
  • Sequence similaritiesBelongs to the kazrin family.
    Contains 3 SAM (sterile alpha motif) domains.
  • Cellular localizationCytoplasm > cytoskeleton and Cytoplasm. Cell junction > desmosome. Nucleus. Observed at the apical plasma mebrane of keratinocytes. Partially colocalizes with PPL and DP at desmosomes, and with PP at the interdesmosomal plasma membrane. Colocalizes with cortical actin-based membrane structures.
  • Information by UniProt
  • Database links
  • Alternative names
    • HRIHFB2003 antibody
    • KAZ antibody
    • Kazn antibody
    • Kazrin antibody
    • KAZRN_HUMAN antibody
    • KIAA1026 antibody
    • RP1-21O18.1 antibody
    see all

Anti-Kazrin antibody images

  • Anti-Kazrin antibody (ab88752) at 1 µg/ml + Rat brain tissue lysate at 50ug/lane

    Predicted band size : 86 kDa
    Observed band size : 86 kDa
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-Kazrin antibody (ab88752) at 1 µg/ml

    Lane 1 : Kazrin transfected 293T cell lysate (45.65 kDa) at 25ug/lane
    Lane 2 : Non-transfected 293T cell lysate at 25ug/lane


    Predicted band size : 86 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

References for Anti-Kazrin antibody (ab88752)

ab88752 has not yet been referenced specifically in any publications.

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