Anti-Kazrin antibody (ab88752)
Key features and details
- Mouse polyclonal to Kazrin
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Kazrin antibody
See all Kazrin primary antibodies -
Description
Mouse polyclonal to Kazrin -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant full length protein within Human Kazrin aa 1-450. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- Rat brain tissue lysate and transfected 293T cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 86 kDa.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 86 kDa. |
Target
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Function
Component of the cornified envelope of keratinocytes. May be involved in the interplay between adherens junctions and desmosomes. The function in the nucleus is not known. -
Tissue specificity
Isoform 2, isoform 3 and isoform 4 are expressed in several cell lines including keratinocytes and bladder and epidermoid carcinoma (at protein level). Isoform 2, isoform 3 and isoform 4 are expressed in hair follicle and interfollicular epidermis (at protein level). -
Sequence similarities
Belongs to the kazrin family.
Contains 3 SAM (sterile alpha motif) domains. -
Cellular localization
Cytoplasm > cytoskeleton and Cytoplasm. Cell junction > desmosome. Nucleus. Observed at the apical plasma mebrane of keratinocytes. Partially colocalizes with PPL and DP at desmosomes, and with PP at the interdesmosomal plasma membrane. Colocalizes with cortical actin-based membrane structures. - Information by UniProt
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Database links
- Entrez Gene: 23254 Human
- Entrez Gene: 313672 Rat
- SwissProt: Q674X7 Human
- SwissProt: Q5FWS6 Rat
- Unigene: 368823 Human
- Unigene: 3753 Rat
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Alternative names
- HRIHFB2003 antibody
- KAZ antibody
- Kazn antibody
see all
Images
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Anti-Kazrin antibody (ab88752) at 1 µg/ml + Rat brain tissue lysate at 50ug/lane
Predicted band size: 86 kDa
Observed band size: 86 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands. -
All lanes : Anti-Kazrin antibody (ab88752) at 1 µg/ml
Lane 1 : Kazrin transfected 293T cell lysate (45.65 kDa) at 25ug/lane
Lane 2 : Non-transfected 293T cell lysate at 25ug/lane
Predicted band size: 86 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (0)
ab88752 has not yet been referenced specifically in any publications.