The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Titre using peptide based assay: 1/1562500.
Use a concentration of 0.625 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionRequired for pseudopod elongation in transformed cells. Substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
Tissue specificitySarcomeric muscle.
PathwayProtein modification; protein ubiquitination.
Post-translational modificationsUbiquitinated and probably targeted for proteasome-independent degradation.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Cell projection > pseudopodium. Cell projection > ruffle. Predominantly cytoplasmic but can co-localize with F-actin at the membrane ruffle-like structures at the tips of transformation-specific pseudopodia.