Overview

  • Product name
    Anti-KBTBD10 antibody
  • Description
    Rabbit polyclonal to KBTBD10
  • Tested applications
    Suitable for: IHC-Fr, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide: AGSLYAIGGF AMIQLESKEF APTEVNDIWK YEDDKKEWAG MLKEIRYASG, corresponding to a region within C terminal amino acids 541-590 of Human KBTBD10

  • Positive control
    • Fetal muscle lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab66605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 24268659
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:1562500.

WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Required for pseudopod elongation in transformed cells. Substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
  • Tissue specificity
    Sarcomeric muscle.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 BTB (POZ) domain.
    Contains 5 Kelch repeats.
  • Post-translational
    modifications
    Ubiquitinated and probably targeted for proteasome-independent degradation.
  • Cellular localization
    Cytoplasm. Cytoplasm > cytoskeleton. Cell projection > pseudopodium. Cell projection > ruffle. Predominantly cytoplasmic but can co-localize with F-actin at the membrane ruffle-like structures at the tips of transformation-specific pseudopodia.
  • Information by UniProt
  • Database links
  • Alternative names
    • cb52 antibody
    • fb24f05 antibody
    • fc15e07 antibody
    • Gm112 antibody
    • KBTBA_HUMAN antibody
    • Kbtbd10 antibody
    • Kel like protein 23 antibody
    • Kel-like protein 23 antibody
    • Kelch related protein 1 antibody
    • Kelch repeat and BTB domain-containing protein 10 antibody
    • Kelch-related protein 1 antibody
    • KRP1 antibody
    • MGC143407 antibody
    • Sarcosin antibody
    • sbcb52 antibody
    • wufb24f05 antibody
    • wufc15e07 antibody
    see all

Images

  • Immunohistochemistry (Frozen sections) analysis of human skeletal muscle tissue from control and nemaline myopathy affected individuals labelling KBTBD10 with ab66605. Costaining with sarcomeric markers in longitudinal planes showed that KLHL41 staining predominated over the I-bands of the sarcomere and at perinuclear regions. Scale bars represent 50 μm.

  • Anti-KBTBD10 antibody (ab66605) at 1.25 µg/ml (in 5% skim milk / PBS buffer) + fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 68 kDa
    Observed band size : 68 kDa
    gel concentration: 12%

References

This product has been referenced in:
  • Gupta VA  et al. Identification of KLHL41 Mutations Implicates BTB-Kelch-Mediated Ubiquitination as an Alternate Pathway to Myofibrillar Disruption in Nemaline Myopathy. Am J Hum Genet 93:1108-17 (2013). IHC-Fr ; Mouse, Human . Read more (PubMed: 24268659) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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