Anti-KBTBD7 antibody (ab230126)
Key features and details
- Rabbit polyclonal to KBTBD7
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KBTBD7 antibody -
Description
Rabbit polyclonal to KBTBD7 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human KBTBD7 aa 450 to the C-terminus.
Database link: Q8WVZ9 -
Positive control
- WB: HL-60, A549 and HEK-293T whole cell lysate. IHC-P: Human testis and brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
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IHC-P |
1/20 - 1/200.
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Notes |
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WB
1/1000 - 1/5000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa). |
IHC-P
1/20 - 1/200. |
Target
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Sequence similarities
Contains 1 BTB (POZ) domain.
Contains 5 Kelch repeats. - Information by UniProt
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Database links
- Entrez Gene: 84078 Human
- GenBank: NM_032138 Human
- SwissProt: Q8WVZ9 Human
- Unigene: 63841 Human
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Alternative names
- 1110008P08Rik antibody
- AU045792 antibody
- DKFZP434E2318 antibody
see all
Images
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All lanes : Anti-KBTBD7 antibody (ab230126) at 1/1000 dilution
Lane 1 : HL-60 (Human promyelocytic leukemia cell line) whole cell lysate
Lane 2 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 77 kDa -
Paraffin-embedded human testis tissue stained for KBTBD7 with ab230126 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human brain tissue stained for KBTBD7 with ab230126 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab230126 has been referenced in 1 publication.
- Akopian D et al. Co-adaptor driven assembly of a CUL3 E3 ligase complex. Mol Cell 82:585-597.e11 (2022). PubMed: 35120648