Overview

  • Product name
  • Description
    Rabbit polyclonal to KCC2
  • Tested applications
    Suitable for: IHC-Fr, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Dog
    Predicted to work with: a wide range of other species
  • Immunogen

    N-terminal His-tag fusion protein corresponding to residues 932-1043 of rat K+/Cl- Cotransporter (KCC2).

  • Positive control
    • Rat brain membrane preparation; Madin Darby Canine Kidney (MDCK) cells

Properties

Applications

Our Abpromise guarantee covers the use of ab49917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 123 kDa.
ICC/IF 1/200 - 1/500. PubMed: 23440186

Target

  • Function
    Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons.
  • Tissue specificity
    Brain specific. Detected in neuronal cells.
  • Sequence similarities
    Belongs to the SLC12A transporter family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Electroneutral potassium chloride cotransporter 2 antibody
    • Electroneutral potassium-chloride cotransporter 2 antibody
    • Erythroid K Cl cotransporter 2 antibody
    • Furosemide sensitive K Cl cotransporter antibody
    • hKCC2 antibody
    • K-Cl cotransporter 2 antibody
    • KCC 2 antibody
    • KCC2 antibody
    • KIAA1176 antibody
    • Neuronal K Cl cotransporter antibody
    • Neuronal K-Cl cotransporter antibody
    • Potassium Chloride Cotransporter antibody
    • Potassium chloride transporter 5 antibody
    • rKCC2 antibody
    • S12A5 antibody
    • S12A5_HUMAN antibody
    • SLC12A5 antibody
    • Solute carrier family 12 (potassium chloride transporter) member 5 antibody
    • Solute carrier family 12 member 5 antibody
    see all

Anti-KCC2 antibody images

  • ab49917 staining KCC2 in rat spinal cord tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with paraformaldehyde and permeabilized using 0.1% Triton-X100. Samples were then blocked with 2% serum for 2 hours at 26°C followed by incubation with the primary antibody, at a 1/4000 dilution, for 19 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 546 (red) used at a 1/4000 dilution.

    See Abreview

  • All lanes : Anti-KCC2 antibody (ab49917) at 1/2000 dilution

    Lane 1 : Whole tissue lysate prepared from chicken auditory brainstem/tectum.
    Lane 2 : Whole tissue lysate prepared from chicken auditory brainstem/tectum, preabsorped with immunogenic peptide.

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 123 kDa


    Exposure time : 10 minutes

    Image courtesy of Tobias Ackels by Abreview.

    See Abreview

References for Anti-KCC2 antibody (ab49917)

This product has been referenced in:
  • Miceli S  et al. Reduced Inhibition within Layer IV of Sert Knockout Rat Barrel Cortex is Associated with Faster Sensory Integration. Cereb Cortex 27:933-949 (2017). Read more (PubMed: 28158484) »
  • Yeo M  et al. Bisphenol A delays the perinatal chloride shift in cortical neurons by epigenetic effects on the Kcc2 promoter. Proc Natl Acad Sci U S A 110:4315-20 (2013). ICC/IF ; Mouse . Read more (PubMed: 23440186) »

See all 4 Publications for this product

Product Wall



The image on the datasheet is from a abreview that was written by a customer.

It shows that with the blocking peptide that blocks all specific binding both bands disappear. This means that both bands depict the KCC2 protein.
Read More

Gracias por contactarnos de nuevo.

Siento mucho los problemas ocasionados por estos productos que no han funcionado como se esperaba.

Para poder dar el soporte oportuno de los mismos y entender por qué han dado ese resultado,...

Read More

Thank you for the protocol details. I think the protocol is generally good, but I am concerned about the length of the post-fixation, 7 days. The perfusion, followed by 4 hours at most for post-fixation, should be sufficient for preservation of the bra...

Read More

Thank you for contacting us.

Are you trying to detect NKCC2 or KCC2?

NKCC2 (SLC12a1) moves one sodium ion, one potassium ion and 2 chloride ions together. KCC2 (SLC12a5) moves two potassiums with 2 chlorides. Because of the differ...

Read More

Thank you very much for your interest in our antibodies. For c-Fos, I would recommend either ab7963 or ab87655.  Both of these antibodies will be guaranteed for use in IHC on mouse samples.  For Aromatase, ab18995 and ab35604 will be guaranteed for you...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Chicken Tissue lysate - whole (Auditory brainstem/Tectum)
Loading amount
20 µg
Specification
Auditory brainstem/Tectum
Gel Running Conditions
Reduced Denaturing (7,5% Acrylamid)
Blocking step
The Blocking Solution (Candor Bioscience) as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
Username

Ms. Stefanie Kurth

Verified customer

Submitted Feb 10 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (spinal cord tissue)
Specification
spinal cord tissue
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X100
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 26°C
Username

Abcam user community

Verified customer

Submitted Sep 23 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Chicken Tissue lysate - other (Brain tissue)
Loading amount
40 µg
Specification
Brain tissue
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Ms. Yuhua Liu

Verified customer

Submitted Feb 09 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Chicken Tissue lysate - other (brain tissue)
Loading amount
40 µg
Specification
brain tissue
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10%, seperate gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Ms. Wei Shi

Verified customer

Submitted Aug 25 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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