Overview

  • Product name
  • Description
    Rabbit polyclonal to KCC2
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Cow, Dog, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human KCC2.

  • Positive control
    • This antibody gave a positive signal in the following rat tissue lysates: brain; cortex; hippocampus.

Properties

Applications

Our Abpromise guarantee covers the use of ab97502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 126 kDa (predicted molecular weight: 126 kDa).

Target

  • Function
    Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons.
  • Tissue specificity
    Brain specific. Detected in neuronal cells.
  • Sequence similarities
    Belongs to the SLC12A transporter family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Electroneutral potassium chloride cotransporter 2 antibody
    • Electroneutral potassium-chloride cotransporter 2 antibody
    • Erythroid K Cl cotransporter 2 antibody
    • Furosemide sensitive K Cl cotransporter antibody
    • hKCC2 antibody
    • K-Cl cotransporter 2 antibody
    • KCC 2 antibody
    • KCC2 antibody
    • KIAA1176 antibody
    • Neuronal K Cl cotransporter antibody
    • Neuronal K-Cl cotransporter antibody
    • Potassium Chloride Cotransporter antibody
    • Potassium chloride transporter 5 antibody
    • rKCC2 antibody
    • S12A5 antibody
    • S12A5_HUMAN antibody
    • SLC12A5 antibody
    • Solute carrier family 12 (potassium chloride transporter) member 5 antibody
    • Solute carrier family 12 member 5 antibody
    see all

Images

  • All lanes : Anti-KCC2 antibody (ab97502) at 1 µg/ml

    Lane 1 : Brain (Rat) Tissue Lysate
    Lane 2 : Rat Cortex Tissue Lysate
    Lane 3 : Rat Hippocampus Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 126 kDa
    Observed band size : 126 kDa
    Additional bands at : 250 kDa,30 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
  • ICC/IF image of ab97502 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97502, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab97502 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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