Overview

  • Product name
    Anti-KCC4 antibody
    See all KCC4 primary antibodies
  • Description
    Goat polyclonal to KCC4
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit
  • Immunogen

    Synthetic peptide:

    C-QLIHDRNTASHT

    , corresponding to internal sequence amino acids 951-962 of Human KCC4 (NP_006589.2).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

Applications

Our Abpromise guarantee covers the use of ab78912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/2,000.
    WB: Preliminary experiments in Human Heart and Kidney lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function
      Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May mediate K(+) uptake into Deiters' cells in the cochlea and contribute to K(+) recycling in the inner ear. Important for the survival of cochlear outer and inner hair cells and the maintenance of the organ of Corti. May be required for basolateral Cl(-) extrusion in the kidney and contribute to renal acidification.
    • Tissue specificity
      Detected in muscle, brain, lung, heart and kidney.
    • Sequence similarities
      Belongs to the SLC12A transporter family.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • D13Ertd261e antibody
      • Electroneutral potassium-chloride cotransporter 4 antibody
      • Furosemide sensitive KCl cotransporter 4 antibody
      • K Cl cotransporter KCC4 antibody
      • K-Cl cotransporter 4 antibody
      • Potassium/chloride transporter KCC4 antibody
      • S12A7_HUMAN antibody
      • SLC12A7 antibody
      • Solute carrier family 12 (potassium/chloride transporters), member 7 antibody
      • Solute carrier family 12 member 7 antibody
      • Solute carrier family 12, member 7 antibody
      see all

    References

    This product has been referenced in:
    • Sun Y  et al. A gel-based proteomic method reveals several protein pathway abnormalities in fetal Down syndrome brain. J Proteomics 74:547-57 (2011). WB ; Human . Read more (PubMed: 21262400) »

    See 1 Publication for this product

    Publishing research using ab78912? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1 Review or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-KCC4 antibody

     Average
    Abreviews
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Hippocampus)
    Loading amount
    5 µg
    Specification
    Hippocampus
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
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    Verified customer

    Submitted Sep 06 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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