- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-KCC4 antibody
See all KCC4 primary antibodies -
DescriptionGoat polyclonal to KCC4
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit
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Immunogen
Synthetic peptide:
C-QLIHDRNTASHT
, corresponding to internal sequence amino acids 951-962 of Human KCC4 (NP_006589.2).
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab78912 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab78912 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Application notes
Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/2,000.
WB: Preliminary experiments in Human Heart and Kidney lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Preliminary experiments in Human Heart and Kidney lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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FunctionMediates electroneutral potassium-chloride cotransport when activated by cell swelling. May mediate K(+) uptake into Deiters' cells in the cochlea and contribute to K(+) recycling in the inner ear. Important for the survival of cochlear outer and inner hair cells and the maintenance of the organ of Corti. May be required for basolateral Cl(-) extrusion in the kidney and contribute to renal acidification.
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Tissue specificityDetected in muscle, brain, lung, heart and kidney.
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Sequence similaritiesBelongs to the SLC12A transporter family.
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Cellular localizationCell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 10723 Human
- Entrez Gene: 20499 Mouse
- Entrez Gene: 100008847 Rabbit
- Entrez Gene: 308069 Rat
- Omim: 604879 Human
- SwissProt: Q9Y666 Human
- SwissProt: Q9WVL3 Mouse
- SwissProt: Q7YRU6 Rabbit
see all -
Alternative names
- D13Ertd261e antibody
- Electroneutral potassium-chloride cotransporter 4 antibody
- Furosemide sensitive KCl cotransporter 4 antibody
see all
Protocols
References for Anti-KCC4 antibody (ab78912)
This product has been referenced in:
- Sun Y et al. A gel-based proteomic method reveals several protein pathway abnormalities in fetal Down syndrome brain. J Proteomics 74:547-57 (2011). WB ; Human . Read more (PubMed: 21262400) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"