Overview

  • Product nameAnti-KCC4 antibody
    See all KCC4 primary antibodies
  • Description
    Goat polyclonal to KCC4
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit
  • Immunogen

    Synthetic peptide:

    C-QLIHDRNTASHT

    , corresponding to internal sequence amino acids 951-962 of Human KCC4 (NP_006589.2).

Properties

Applications

Our Abpromise guarantee covers the use of ab78912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesPeptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/2,000.
    WB: Preliminary experiments in Human Heart and Kidney lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMediates electroneutral potassium-chloride cotransport when activated by cell swelling. May mediate K(+) uptake into Deiters' cells in the cochlea and contribute to K(+) recycling in the inner ear. Important for the survival of cochlear outer and inner hair cells and the maintenance of the organ of Corti. May be required for basolateral Cl(-) extrusion in the kidney and contribute to renal acidification.
    • Tissue specificityDetected in muscle, brain, lung, heart and kidney.
    • Sequence similaritiesBelongs to the SLC12A transporter family.
    • Cellular localizationCell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • D13Ertd261e antibody
      • Electroneutral potassium-chloride cotransporter 4 antibody
      • Furosemide sensitive KCl cotransporter 4 antibody
      • K Cl cotransporter KCC4 antibody
      • K-Cl cotransporter 4 antibody
      • Potassium/chloride transporter KCC4 antibody
      • S12A7_HUMAN antibody
      • SLC12A7 antibody
      • Solute carrier family 12 (potassium/chloride transporters), member 7 antibody
      • Solute carrier family 12 member 7 antibody
      • Solute carrier family 12, member 7 antibody
      see all

    References for Anti-KCC4 antibody (ab78912)

    This product has been referenced in:
    • Sun Y  et al. A gel-based proteomic method reveals several protein pathway abnormalities in fetal Down syndrome brain. J Proteomics 74:547-57 (2011). WB ; Human . Read more (PubMed: 21262400) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Sample Mouse Tissue lysate - whole (Hippocampus)
    Loading amount 5 µg
    Specification Hippocampus
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 06 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"