Overview

  • Product nameAnti-KChIP2 antibody [S60-73]
    See all KChIP2 primary antibodies
  • Description
    Mouse monoclonal [S60-73] to KChIP2
  • SpecificityNo cross-reactivity is observed against Rat KCNIP1 and KCNIP3
  • Tested applicationsSuitable for: WB, IHC-P, IHC-Fr, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1 - 252 of Rat KChIP2 isoform 2 (accession number AAF81756).

  • Positive control
    • Mouse brain lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab99041 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa.
IHC-P Use a concentration of 0.1 - 1 µg/ml. (perox) or 1 - 10µg/mL (IF).
IHC-Fr Use a concentration of 0.1 - 1 µg/ml. (perox) or 1 - 10µg/mL (IF).
ICC/IF Use a concentration of 0.1 - 1 µg/ml. (perox) or 1 - 10µg/mL (IF).
Flow Cyt Use 0.1-1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionRegulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels. Modulates channel density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner. In vitro, modulates KCND2/Kv4.2 and KCND3/Kv4.3 currents. Involved in KCND2 and KCND3 trafficking to the cell surface. May be required for the expression of I(To) currents in the heart.
  • Tissue specificityExpressed in brain. Colocalizes with KCND2 in excitatory neurons including cortical and hippocampal CA1 pyramidal cells. Isoform 3 is expressed in heart and in umbilical vein endothelial cells. Not expressed in fetal heart.
  • Sequence similaritiesBelongs to the recoverin family.
    Contains 4 EF-hand domains.
  • Post-translational
    modifications
    Palmitoylated. Palmitoylation enhances association with the plasma membrane.
  • Cellular localizationCell membrane and Cell membrane. Detected on lipid rafts (By similarity).
  • Information by UniProt
  • Database links
  • FormThere are 9 isoforms produced by alternative splicing.
  • Alternative names
    • A type potassium channel modulatory protein 2 antibody
    • A-type potassium channel modulatory protein 2 antibody
    • Cardiac voltage gated potassium channel modulatory subunit antibody
    • Cardiac voltage-gated potassium channel modulatory subunit antibody
    • DKFZp566L1246 antibody
    • KChIP 2 antibody
    • KChIP2 antibody
    • KCIP2_HUMAN antibody
    • KCNIP 2 antibody
    • Kcnip2 antibody
    • Kv channel interacting protein 2 antibody
    • Kv channel-interacting protein 2 antibody
    • MGC17241 antibody
    • Potassium channel interacting protein 2 antibody
    • Potassium channel-interacting protein 2 antibody
    see all

Anti-KChIP2 antibody [S60-73] images

  • Overlay histogram showing SH-SH5Y cells stained with ab99041 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab99041, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Immunohistochemical detection of KChIP2 in frozen Mouse brain sections using ab99041.


  • Predicted band size : 29 kDa
    Western blot detection of KChip2 in Rat brain membrane tissues using ab99041 at 1:1000.

References for Anti-KChIP2 antibody [S60-73] (ab99041)

This product has been referenced in:
  • Panguluri SK  et al. Hyperoxia-induced hypertrophy and ion channel remodeling in left ventricle. Am J Physiol Heart Circ Physiol 304:H1651-61 (2013). Mouse . Read more (PubMed: 23585127) »

See 1 Publication for this product

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