Overview

  • Product name
    Anti-KCNAB3 antibody
  • Description
    Rabbit polyclonal to KCNAB3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A region within synthetic peptide: GRGTGMKYRN LGKSGLRVSC LGLGTWVTFG SQISDETAED VLTVAYEHGV, corresponding to N terminal amino acids 73-122 of Human KCNAB3

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Human kidney tissue, Jurkat cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.65 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Function
    Accessory potassium channel protein which modulates the activity of the pore-forming alpha subunit. Alters the functional properties of Kv1.5.
  • Tissue specificity
    Brain specific. Most prominent expression in cerebellum. Weaker signals detected in cortex, occipital lobe, frontal lobe and temporal lobe. Not detected in spinal cord, heart, lung, liver, kidney, pancreas, placenta and skeletal muscle.
  • Sequence similarities
    Belongs to the shaker potassium channel beta subunit family.
  • Domain
    Alteration of functional properties of alpha subunit is mediated through N-terminal domain of beta subunit.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • AKR6A9 antibody
    • K antibody
    • K(+) channel subunit beta-3 antibody
    • K+ channel beta 3 subunit antibody
    • KCAB3_HUMAN antibody
    • KCNA3.1B antibody
    • KCNA3B antibody
    • KCNAB 3 antibody
    • Kcnab3 antibody
    • KV BETA 3 antibody
    • KV BETA3 antibody
    • Kv-beta-3 antibody
    • MGC116886 antibody
    • Voltage gated potassium channel subunit beta 3 antibody
    • Voltage-gated potassium channel subunit beta-3 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KCNAB3 antibody (ab66625)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KCNAB3 antibody (ab66625)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling KCNAB3 with ab66625 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubules. Magnification: 400X.

  • Western blot - KCNAB3 antibody (ab66625)
    Western blot - KCNAB3 antibody (ab66625)
    Anti-KCNAB3 antibody (ab66625) at 0.65 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 44 kDa
    Observed band size: 44 kDa
    Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.

References

ab66625 has not yet been referenced specifically in any publications.

Publishing research using ab66625? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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