Overview

  • Product name
  • Description
    Rabbit polyclonal to KCNC1
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A region within a synthetic peptide: LAEPDAHSHF DYDPRADEFF FDRHPGVFAH ILNYYRTGKL HCPADVCGPL, corresponding to N terminal amino acids 37-86 of Human KCNC1

  • Positive control
    • HepG2 cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.

WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 58 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient. Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCNC2, and possibly other family members as well. Contributes to fire sustained trains of very brief action potentials at high frequency in pallidal neurons.
  • Involvement in disease
    Epilepsy, progressive myoclonic 7
  • Sequence similarities
    Belongs to the potassium channel family. C (Shaw) (TC 1.A.1.2) subfamily. Kv3.1/KCNC1 sub-subfamily.
  • Domain
    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
    The tail may be important in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C230009H10Rik antibody
    • FLJ41162 antibody
    • FLJ42249 antibody
    • FLJ43491 antibody
    • Kcnc1 antibody
    • KCNC1_HUMAN antibody
    • Kcr2 1 antibody
    • KShIIIB antibody
    • Kv3.1 antibody
    • Kv4 antibody
    • MGC129855 antibody
    • NGK2 antibody
    • Potassium voltage-gated channel subfamily C member 1 antibody
    • Shaw antibody
    • Voltage gated potassium channel antibody
    • Voltage gated potassium channel subunit Kv3.1 antibody
    • Voltage-gated potassium channel subunit Kv3.1 antibody
    • Voltage-gated potassium channel subunit Kv4 antibody
    see all

Images

  • Anti-KCNC1 antibody (ab65535) at 2.5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 58 kDa

References

This product has been referenced in:
  • Bocksteins E  et al. Kv3 channels contribute to the delayed rectifier current in small cultured mouse dorsal root ganglion neurons. Am J Physiol Cell Physiol 303:C406-15 (2012). WB, ICC/IF ; Mouse . Read more (PubMed: 22673617) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for contacting us.
Though these kits has not been tested with rat samples however based on the material used in these kits these will compatible with all type of mammalian cells.
I can confirm that these kits will be suitable for Ra...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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