Overview

  • Product name
  • Description
    Rabbit polyclonal to KCNE3
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Hamster, Monkey, Gorilla, Bat
  • Immunogen

    Synthetic peptide corresponding to Human KCNE3 (internal sequence). The peptide corresponds to 18 amino acids. (NP_005463.1)
    Database link: Q9Y6H6

  • Positive control
    • Human kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab189022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Relevance
    KCNE3 (also known as MiRP2) is an ancillary protein that functions as a beta subunit with a voltage-gated potassium channel complex of pore-forming alpha subunits. It modulates the gating kinetics of the complex, as well as enhancing channel stability. The complex of KCNE3 with KCNQ1/KCLQT1 is postulated form the intestinal cAMP-stimulated potassium channel that is involved in chloride secretion. KCNE3 in complex with KCNC4/Kv3.4 is thought to form the subthreshold voltage-gated potassium channel that establishes the resting membrane potential in skeletal muscle. Altered activity of the KCNE3/Kv3.4 channel induced by the Abeta peptide is thought to result in the upregulation of the transient outward potassium current, a significant step in the etiology of Alzheimer's disease.
  • Cellular localization
    Cell Membrane
  • Database links
  • Alternative names
    • Cardiac voltage gated potassium channel accessory subunit antibody
    • HOKPP antibody
    • KCNE 3 antibody
    • Minimum potassium ion channel related peptide 2 antibody
    • minK related peptide 2 antibody
    • MiRP 2 antibody
    • MiRP2 antibody
    • Potassium voltage gated channel subfamily E member 3 antibody
    • Potassium voltage gated channel, Isk related family, member 3 antibody
    • Voltage gated K+ channel subunit MIRP2 antibody
    see all

Images

  • Immunohistochemistry of formalin-fixed, paraffin-embedded human kidney tissue labelling KCNE3 with ab189022 at 5 µg/ml.

References

ab189022 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Duodenum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako retrieval buffer (pH9) in a steamer
Permeabilization
No
Specification
Duodenum
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 20% · Temperature: 22°C
Fixative
Formaldehyde
Username

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Submitted Mar 14 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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