Overview

  • Product nameAnti-KCNH1 antibody
    See all KCNH1 primary antibodies
  • Description
    Rabbit polyclonal to KCNH1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 850 - 950 of Rat KCNH1.

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Rat Hippocampus; Rat Whole Brain; Mouse Hippocampus; Mouse Whole Brain.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab101174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).

Target

  • FunctionPore-forming (alpha) subunit of voltage-gated non-inactivating delayed rectifier potassium channel. Channel properties may be modulated by cAMP and subunit assembly. Mediates IK(NI) current in myoblasts.
  • Tissue specificityHighly expressed in brain and in myoblasts at the onset of fusion, but not in other tissues. Detected in HeLa (cervical carcinoma), SH-SY5Y (neuroblastoma) and MCF-7 (epithelial tumor) cells, but not in normal epithelial cells.
  • Sequence similaritiesBelongs to the potassium channel family. H (Eag) (TC 1.A.1.20) subfamily. Kv10.1/KCNH1 sub-subfamily.
    Contains 1 cyclic nucleotide-binding domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 1 PAS (PER-ARNT-SIM) domain.
  • DomainThe segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EAG antibody
    • EAG channel 1 antibody
    • EAG1 antibody
    • Ether a go go potassium channel 1 antibody
    • Ether a go go, Drosophila, homolog of antibody
    • Ether-a-go-go potassium channel 1 antibody
    • ether-a-go-go, Drosophila, homolog of antibody
    • h eag antibody
    • h-eag antibody
    • hEAG1 antibody
    • Kcnh1 antibody
    • KCNH1_HUMAN antibody
    • Kv10.1 antibody
    • M eag antibody
    • MGC124419 antibody
    • MGC124420 antibody
    • MGC142269 antibody
    • Potassium voltage gated channel subfamily H (eag related), member 1 antibody
    • Potassium voltage-gated channel subfamily H member 1 antibody
    • Voltage gated potassium channel subunit Kv10.1 antibody
    • Voltage-gated potassium channel subunit Kv10.1 antibody
    see all

Anti-KCNH1 antibody images

  • All lanes : Anti-KCNH1 antibody (ab101174) at 1 µg/ml

    Lane 1 : Rat Hippocampus Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : Mouse Hippocampus Tissue Lysate
    Lane 4 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 108 kDa
    Observed band size : 108 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

References for Anti-KCNH1 antibody (ab101174)

ab101174 has not yet been referenced specifically in any publications.

Product Wall

Vielen Dank für diese Rückmeldung. Es tut mir wirklich sehr leid, dass Sie keine zufriedenstellenden Ergebnisse erhalten haben! Zu den KCNH5-Antikörpern: Es ist in der Tat sehr einfach, in den 'Additional Notes' eine kurze Notiz zu den erzielten Ergebn...

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