Overview

  • Product name
  • Description
    Rabbit polyclonal to KCNH1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 850 - 950 of Rat KCNH1.

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Rat Hippocampus; Rat Whole Brain; Mouse Hippocampus; Mouse Whole Brain.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab101174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).

Target

  • Function
    Pore-forming (alpha) subunit of voltage-gated non-inactivating delayed rectifier potassium channel. Channel properties may be modulated by cAMP and subunit assembly. Mediates IK(NI) current in myoblasts.
  • Tissue specificity
    Highly expressed in brain and in myoblasts at the onset of fusion, but not in other tissues. Detected in HeLa (cervical carcinoma), SH-SY5Y (neuroblastoma) and MCF-7 (epithelial tumor) cells, but not in normal epithelial cells.
  • Sequence similarities
    Belongs to the potassium channel family. H (Eag) (TC 1.A.1.20) subfamily. Kv10.1/KCNH1 sub-subfamily.
    Contains 1 cyclic nucleotide-binding domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 1 PAS (PER-ARNT-SIM) domain.
  • Domain
    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EAG antibody
    • EAG channel 1 antibody
    • EAG1 antibody
    • Ether a go go potassium channel 1 antibody
    • Ether a go go, Drosophila, homolog of antibody
    • Ether-a-go-go potassium channel 1 antibody
    • ether-a-go-go, Drosophila, homolog of antibody
    • h eag antibody
    • h-eag antibody
    • hEAG1 antibody
    • Kcnh1 antibody
    • KCNH1_HUMAN antibody
    • Kv10.1 antibody
    • M eag antibody
    • MGC124419 antibody
    • MGC124420 antibody
    • MGC142269 antibody
    • Potassium voltage gated channel subfamily H (eag related), member 1 antibody
    • Potassium voltage gated channel subfamily H member 1 antibody
    • Potassium voltage-gated channel subfamily H member 1 antibody
    • Voltage gated potassium channel subunit Kv10.1 antibody
    • Voltage-gated potassium channel subunit Kv10.1 antibody
    see all

Images

  • All lanes : Anti-KCNH1 antibody (ab101174) at 1 µg/ml

    Lane 1 : Rat Hippocampus Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : Mouse Hippocampus Tissue Lysate
    Lane 4 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 108 kDa
    Observed band size : 108 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

References

ab101174 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Vielen Dank für diese Rückmeldung. Es tut mir wirklich sehr leid, dass Sie keine zufriedenstellenden Ergebnisse erhalten haben! Zu den KCNH5-Antikörpern: Es ist in der Tat sehr einfach, in den 'Additional Notes' eine kurze Notiz zu den erzielten Ergebn...

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