• Product nameAnti-KCNJ1 antibody
    See all KCNJ1 primary antibodies
  • Description
    Goat polyclonal to KCNJ1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide:


    , corresponding to internal sequence amino acids 245-259 of Human KCNJ1 (NP_000211.1; NP_722448.1)

  • Positive control
    • Human Kidney lysate



Our Abpromise guarantee covers the use of ab92285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesWB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 45 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionIn the kidney, probably plays a major role in potassium homeostasis. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This channel is activated by internal ATP and can be blocked by external barium.
    • Tissue specificityIn the kidney and pancreatic islets. Lower levels in skeletal muscle, pancreas, spleen, brain, heart and liver.
    • Involvement in diseaseDefects in KCNJ1 are the cause of Bartter syndrome type 2 (BS2) [MIM:241200]; also termed hyperprostanglandin E syndrome 2. BS refers to a group of autosomal recessive disorders characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. BS2 is a life-threatening condition beginning in utero, with marked fetal polyuria that leads to polyhydramnios and premature delivery. Another hallmark of BS2 is a marked hypercalciuria and, as a secondary consequence, the development of nephrocalcinosis and osteopenia.
    • Sequence similaritiesBelongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ1 subfamily.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATP regulated potassium channel ROM K antibody
      • ATP sensitive inward rectifier potassium channel 1 antibody
      • ATP-regulated potassium channel ROM-K antibody
      • ATP-sensitive inward rectifier potassium channel 1 antibody
      • Inward rectifier K(+) channel Kir1.1 antibody
      • inwardly rectifying K+ channel antibody
      • inwardly rectifying subfamily J member 1 antibody
      • IRK1_HUMAN antibody
      • KCNJ 1 antibody
      • KCNJ antibody
      • Kcnj1 antibody
      • Kir 1.1 antibody
      • Kir1.1 antibody
      • OTTHUMP00000045938 antibody
      • Potassium channel antibody
      • Potassium channel inwardly rectifying subfamily J member 1 antibody
      • potassium inwardly-rectifying channel J1 antibody
      • ROMK 1 antibody
      • ROMK 2 antibody
      • ROMK antibody
      • ROMK1 antibody
      • ROMK2 antibody
      see all

    Anti-KCNJ1 antibody images

    • Anti-KCNJ1 antibody (ab92285) at 1 µg/ml + Human Kidney lysate at 35 µg
      developed using the ECL technique

      Predicted band size : 45 kDa
      Observed band size : 45 kDa

    References for Anti-KCNJ1 antibody (ab92285)

    ab92285 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Rat Tissue lysate - whole (Heart)
    Gel Running Conditions Reduced Denaturing
    Loading amount 40 µg
    Specification Heart
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Apr 27 2015