Anti-KCNJ1 (phospho S44/25) antibody (ab111489)


  • Product nameAnti-KCNJ1 (phospho S44/25) antibody
    See all KCNJ1 primary antibodies
  • Description
    Rabbit polyclonal to KCNJ1 (phospho S44/25)
  • Specificityab111489 detects endogenous levels of KCNJ1 only when phosphorylated at serine 44/25 (Human: Ser44; Mouse: Ser25; Rat: Ser44).
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from Human KCNJ1 around the phosphorylation site of serine 44/25 (L-V-SP-K-D).

  • Positive control
    • A549 cells; Human brain tissue.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 49% PBS, 0.88% Sodium chloride
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab111489 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab111489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.


  • FunctionIn the kidney, probably plays a major role in potassium homeostasis. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This channel is activated by internal ATP and can be blocked by external barium.
  • Tissue specificityIn the kidney and pancreatic islets. Lower levels in skeletal muscle, pancreas, spleen, brain, heart and liver.
  • Involvement in diseaseDefects in KCNJ1 are the cause of Bartter syndrome type 2 (BS2) [MIM:241200]; also termed hyperprostanglandin E syndrome 2. BS refers to a group of autosomal recessive disorders characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. BS2 is a life-threatening condition beginning in utero, with marked fetal polyuria that leads to polyhydramnios and premature delivery. Another hallmark of BS2 is a marked hypercalciuria and, as a secondary consequence, the development of nephrocalcinosis and osteopenia.
  • Sequence similaritiesBelongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ1 subfamily.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP regulated potassium channel ROM K antibody
    • ATP sensitive inward rectifier potassium channel 1 antibody
    • ATP-regulated potassium channel ROM-K antibody
    • ATP-sensitive inward rectifier potassium channel 1 antibody
    • Inward rectifier K(+) channel Kir1.1 antibody
    • inwardly rectifying K+ channel antibody
    • inwardly rectifying subfamily J member 1 antibody
    • IRK1_HUMAN antibody
    • KCNJ 1 antibody
    • KCNJ antibody
    • Kcnj1 antibody
    • Kir 1.1 antibody
    • Kir1.1 antibody
    • OTTHUMP00000045938 antibody
    • Potassium channel antibody
    • Potassium channel inwardly rectifying subfamily J member 1 antibody
    • potassium inwardly-rectifying channel J1 antibody
    • ROMK 1 antibody
    • ROMK 2 antibody
    • ROMK antibody
    • ROMK1 antibody
    • ROMK2 antibody
    see all

Anti-KCNJ1 (phospho S44/25) antibody images

  • ab111489, at 1/50 dilution, staining KCNJ1 in paraffin-embedded Human brain tissue by Immunohistochemistry, in the presence (right panel) or absence (left panel) of immunising peptide.
  • ab111489, at 1/100 dilution, staining KCNJ1 in A549 cells by Immunofluorescence, in the presence (right panel) or absence (left panel) of immunising peptide.

References for Anti-KCNJ1 (phospho S44/25) antibody (ab111489)

ab111489 has not yet been referenced specifically in any publications.

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