Anti-KCNK18/TRESK antibody (ab96868)

Overview

• Product name
  Anti-KCNK18/TRESK antibody
  See all KCNK18/TRESK primary antibodies
• Description
  Rabbit polyclonal to KCNK18/TRESK
• Tested applications
  Suitable for: WBmore details
• Species reactivity
  Reacts with: Mouse, Rat
  
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Mouse KCNK18/TRESK.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab109923.)

• Positive control
  • This antibody gave a positive signal in the following tissue lysates: Mouse forebrain; Mouse cerebellum; Mouse hippocampus; Mouse spinal cord; Mouse brain; Rat brain; Rat cortex.

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
• Storage buffer
  Preservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• Purity
  Immunogen affinity purified
• Clonality
  Polyclonal
• Isotype
  IgG
• Research areas
  • Neuroscience
  • Neurotransmission
  • Receptors / Channels
  • Potassium Channels

  • Neuroscience
  • Neurotransmission
  • Receptors / Channels
  • GPCR
  • More GPCR

  • Signal Transduction
  • Signaling Pathway
  • G Protein Signaling
  • GPCR

Associated products

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

Applications

Target

• Function
  Outward rectifying potassium channel. Produces rapidly activating outward rectifier K(+) currents. May function as background potassium channel that sets the resting membrane potential. Channel activity is directly activated by calcium signal. Activated by the G(q)-protein coupled receptor pathway. The calcium signal robustly activates the channel via calcineurin, whereas the anchoring of 14-3-3/YWHAH interferes with the return of the current to the resting state after activation. Inhibited also by arachidonic acid and other naturally occurring unsaturated free fatty acids. Channel activity is also enhanced by volatile anesthetics, such as isoflurane. Appears to be the primary target of hydroxy-alpha-sanshool, an ingredient of Schezuan pepper. May be involved in the somatosensory function with special respect to pain sensation.
• Tissue specificity
  Expressed specifically in dorsal root ganglion and trigeminal ganglion neurons. Detected at low levels in spinal cord.
• Involvement in disease
  Defects in KCNK18 are a cause of migraine with or without aura type 13 (MGR13) [MIM:613656]. A form of migraine trasmitted in an autosomal dominant pattern. Migraine is a disabling symptom complex of periodic headaches, usually temporal and unilateral. Headaches are often accompanied by irritability, nausea, vomiting and photobia, preceded by constriction of the cranial arteries. The two major subtypes are common migraine (migraine without aura) and classic migraine (migraine with aura). Classic migraine is characterized by recurrent attacks of reversible neurological symptoms (aura) that precede or accompany the headache. Aura may include a combination of sensory disturbances, such as blurred vision, hallucinations, vertigo, numbness and difficulty in concentrating and speaking. Note=Susceptibility to migraine has been shown to be conferred by a frameshift mutation that segregates with the disorder in a large multigenerational family. Migraine was associated with sensitivity to lights, sounds, and smells, as well as nausea and occasional vomiting. Triggers included fatigue, alcohol and bright lights. Mutations in KCNK18 are a rare cause of migraine.
• Sequence similarities
  Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.
• Post-translational
  modifications
  Phosphorylation of Ser-252 is required for the binding of 14-3-3eta/YWHAH. Calcineurin-mediated dephosphorylation of Ser-264 enhances channel activity.
  N-glycosylated.
• Cellular localization
  Cell membrane.

• Target information above from: UniProt accession Q7Z418 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 332396 Mouse
  • Entrez Gene: 445371 Rat
  • SwissProt: Q6VV64 Mouse
  • SwissProt: Q6Q1P3 Rat
  • Unigene: 329947 Mouse
  • Unigene: 124394 Rat
• Alternative names
  • K2p18.1 antibody
  • KCNK18 antibody
  • KCNKI_HUMAN antibody

  • MGR13 antibody
  • OTTHUMP00000020575 antibody
  • Potassium channel subfamily K member 18 antibody
  • TRESK 2 antibody
  • TRESK antibody
  • TRESK2 antibody
  • TRIK antibody
  • TWIK related individual K+ channel antibody
  • TWIK related individual potassium channel antibody
  • TWIK related spinal cord K+ channel antibody
  • TWIK related spinal cord potassium channel antibody
  • TWIK-related individual potassium channel antibody
  • TWIK-related spinal cord potassium channel antibody

  see all

Protocols

• Western blot protocols