The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/312500.
RelevanceKCNK4 belongs to the two pore domain potassium channel family and is an outward rectifying potassium channel. This channel produces rapidly activating and non-inactivating outward rectifier K(+) currents. It is activated by arachidonic acid and other naturally occurring unsaturated free fatty acids.