Overview

  • Product name
    Anti-KCNMB2 antibody [N53/32]
    See all KCNMB2 primary antibodies
  • Description
    Mouse monoclonal [N53/32] to KCNMB2
  • Specificity
    No cross-reactivity against BKBeta1, BKBeta3 or BKBeta4. Not yet validated for use in mammalian brain.
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein corresponding to amino acids 1 - 41 (MFIWTSGRTS SSYRQDEKRN IYQKIRDHDL LDKRKTVTAL K; N-terminus) and 218 - 235 (KLTQYLSLLC ERIQRINR; C-terminus) of Mouse KCNMB2 (UniProt ID: Q9CZM9).

  • Positive control
    • Lysate from COS-1 cells transfected with KCNMB2
  • General notes

    The clone number has been updated from S53-32 to N53/32, both clone numbers name the same antibody clone.

     

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    N53/32
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab94589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
IHC-Fr
ICC/IF
  • Application notes
    ICC/IF: Use at a concentration of 0.1-1.0µg/mL (perox) or 1.0-10µg/mL (IF).
    IHC-P: Use at a concentration of 0.1-1.0µg/mL (perox) or 1.0-10µg/mL (IF).
    IHC-Fr: Use at a concentration of 0.1-1.0µg/mL (perox) or 1.0-10µg/mL (IF).
    WB: Use at a concentration of 1 - 10 µg/ml. Predicted molecular weight: 27 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Acts as a negative regulator that confers rapid and complete inactivation of KCNMA1 channel complex. May participate in KCNMA1 inactivation in chromaffin cells of the adrenal gland or in hippocampal CA1 neurons.
    • Tissue specificity
      Expressed in kidney, heart and brain. Highly expressed in ovary. Expressed at low level in other tissues.
    • Sequence similarities
      Belongs to the KCNMB (TC 8.A.14.1) family. KCNMB2 subfamily.
    • Domain
      The ball and chain domain mediates the inactivation of KCNMA1. It occludes the conduction pathway of KCNMA1 channels, and comprises the pore-blocking ball domain (residues 1-17) and the chain domain (residues 20-45) linking it to the transmembrane segment. The ball domain is made up of a flexible N-terminus anchored at a well ordered loop-helix motif. The chain domain consists of a 4-turn helix with an unfolded linker at its C-terminus.
    • Post-translational
      modifications
      N-glycosylated.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2700049B16Rik antibody
      • 3110031N04Rik antibody
      • BK channel subunit beta 2 antibody
      • BK channel subunit beta-2 antibody
      • BKbeta2 antibody
      • Calcium activated potassium channel subfamily M subunit beta 2 antibody
      • Calcium activated potassium channel subunit beta 2 antibody
      • Calcium-activated potassium channel antibody
      • Calcium-activated potassium channel subunit beta-2 antibody
      • Charybdotoxin receptor subunit beta 2 antibody
      • Charybdotoxin receptor subunit beta-2 antibody
      • Hbeta2 antibody
      • Hbeta3 antibody
      • K(VCA)beta-2 antibody
      • K(VCA)beta2 antibody
      • KCMB2_HUMAN antibody
      • KCNMB 2 antibody
      • Kcnmb2 antibody
      • Large conductance Ca2+ activated K+ channel beta2 subunit antibody
      • Large conductance calcium activated potassium channel beta 2 subunit antibody
      • Maxi K channel subunit beta 2 antibody
      • Maxi K channel subunit beta-2 antibody
      • MaxiK channel beta 2 subunit antibody
      • MGC22431 antibody
      • MGC57945 antibody
      • Potassium large conductance calcium activated channel subfamily M beta member 2 antibody
      • Slo-beta-2 antibody
      • Slobeta2 antibody
      • subfamily M subunit beta-2 antibody
      see all

    Images



    • Predicted band size : 27 kDa
      Western blot detection of KCNMB2 in a Human cell line mix using ab94589 at 1:1000 dilution.

    References

    ab94589 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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