Overview

  • Product name
    Anti-KCNMB3 antibody [N40B/18]
    See all KCNMB3 primary antibodies
  • Description
    Mouse monoclonal [N40B/18] to KCNMB3
  • Host species
    Mouse
  • Specificity
    No cross-reactivity against BKBeta1, BKBeta3b or BKBeta4.
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein corresponding to amino acids 1-49 (unique N-terminus) of Mouse KCNMB3.

  • Positive control
    • Lysate from COS-1 cells transiently transfected with KCNMB3a.
  • General notes

    The clone number has been updated from S40B-18 to N40B/18, both clone numbers name the same antibody clone.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab94590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
IHC-Fr
ICC/IF
  • Application notes
    ICC/IF: Use at a concentration of 0.1-1.0µg/mL (perox) or 1.0-10µg/mL (IF).
    IHC-P: Use at a concentration of 0.1-1.0µg/mL (perox) or 1.0-10µg/mL (IF).
    IHC-Fr: Use at a concentration of 0.1-1.0µg/mL (perox) or 1.0-10µg/mL (IF).
    WB: Use at a concentration of 1 - 10 µg/ml. Predicted molecular weight: 32 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Alters the functional properties of the current expressed by the KCNMA1 channel. Isoform 2, isoform 3 and isoform 4 partially inactivate the current of KCNBMA. Isoform 4 induces a fast and incomplete inactivation of KCNMA1 channel that is detectable only at large depolarizations. In contrast, isoform 1 does not induce detectable inactivation of KCNMA1. Two or more subunits of KCNMB3 are required to block the KCNMA1 tetramer.
    • Tissue specificity
      Isoform 1, isoform 3 and isoform 4 are widely expressed. Isoform 2 is expressed placenta, pancreas, kidney and heart. Isoform 1 and isoform 3 are highly expressed in pancreas and testis.
    • Sequence similarities
      Belongs to the KCNMB (TC 8.A.14.1) family. KCNMB3 subfamily.
    • Domain
      Isoform 4 cytoplasmic N-terminal domain participates in the partial inactivation of KCNMA1, possibly by binding to a receptor site.
      The extracellular domain forms gates to block ion permeation, providing a mechanism by which current can be rapidly diminished upon cellular repolarization.
    • Post-translational
      modifications
      N-glycosylated.
      The extracellular domain contains disulfide bond essential for the gating mechanism.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • BK channel subunit beta 3 antibody
      • BK channel subunit beta-3 antibody
      • BKbeta 3 antibody
      • BKbeta3 antibody
      • Calcium activated potassium channel beta 3 subunit antibody
      • Calcium activated potassium channel subfamily M subunit beta 3 antibody
      • Calcium activated potassium channel subunit beta 3 antibody
      • Calcium-activated potassium channel antibody
      • Calcium-activated potassium channel subunit beta-3 antibody
      • Charybdotoxin receptor subunit beta 3 antibody
      • Charybdotoxin receptor subunit beta-3 antibody
      • EG435726 antibody
      • Gm5707 antibody
      • Hbeta 3 antibody
      • Hbeta3 antibody
      • K(VCA)beta 3 antibody
      • K(VCA)beta-3 antibody
      • KCMB3_HUMAN antibody
      • KCNMB 2 antibody
      • KCNMB 3 antibody
      • KCNMB2 antibody
      • KCNMB3 antibody
      • KCNMBL antibody
      • Large conductance voltage and Ca2+ activated potassium channel Maxi K beta 3 subunit antibody
      • Maxi K channel subunit beta 3 antibody
      • Maxi K channel subunit beta-3 antibody
      • Potassium channel, calcium-activated large conductance, subfamily M, beta member 3 antibody
      • Potassium large conductance calcium activated channel beta 3 subunit antibody
      • Potassium large conductance calcium activated channel subfamily M beta member 3 antibody
      • Slo beta 3 antibody
      • Slo-beta-3 antibody
      • subfamily M subunit beta-3 antibody
      see all

    Images

    • Predicted band size: 32 kDa



      Western blot detection of KCNMB3 in Rat brain membrane lysates using ab94590 at 1:1000 dilution.

    References

    ab94590 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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