Overview

  • Product name
  • Description
    Rabbit polyclonal to KCNN1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Horse, Cow, Cat, Dog, Pig
  • Immunogen

    A region within synthetic peptide: QAQKLRSVKI EQGKLNDQAN TLTDLAKTQT VMYDLVSELH AQHEELEARL, corresponding to C terminal amino acids 433-482 of Human KCNN1

  • Positive control
    • Jurkat cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 32, 60 kDa (predicted molecular weight: 60 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Forms a voltage-independent potassium channel activated by intracellular calcium. Activation is followed by membrane hyperpolarization. Thought to regulate neuronal excitability by contributing to the slow component of synaptic afterhyperpolarization. The channel is blocked by apamin.
  • Sequence similarities
    Belongs to the potassium channel KCNN family. KCa2.1/KCNN1 subfamily.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • hSK1 antibody
    • KCa2.1 antibody
    • KCNN 1 antibody
    • Kcnn1 antibody
    • KCNN1_HUMAN antibody
    • Potassium intermediate/small conductance calcium activated channel subfamily N member 1 antibody
    • SK antibody
    • SK1 antibody
    • SKCa 1 antibody
    • SKCa1 antibody
    • Small conductance calcium activated potassium channel protein 1 antibody
    • Small conductance calcium-activated potassium channel protein 1 antibody
    see all

Images

  • Anti-KCNN1 antibody (ab66624) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 60 kDa
    Observed band size : 32,60 kDa (why is the actual band size different from the predicted?)

References

ab66624 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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In order to predict crossreactivity of this antibody with the mouse sequence, I have asked the laboratory to provide a sequence alignment with the mouse sequence. Unfortunately the sequence of the immunogen is only to 7...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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