Overview

  • Product nameAnti-KCNN2 antibody
    See all KCNN2 primary antibodies
  • Description
    Rabbit polyclonal to KCNN2
  • SpecificityThis antibody recognizes KCNN2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Zebrafish
  • Immunogen

    A region within synthetic peptide: IDHAKVRKHQ RKFLQAIHQL RSVKMEQRKL NDQANTLVDL AKTQNIMYDM, corresponding to amino acids 443-492 of Human KCNN2

  • Positive control
    • HepG2 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab48817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 64 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay was 1:62500.

Target

Anti-KCNN2 antibody images

  • Anti-KCNN2 antibody (ab48817) at 1.25 µg/ml + HepG2 lysate at 10 µg with 5% skimmed milk in PBS.

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 64 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

References for Anti-KCNN2 antibody (ab48817)

This product has been referenced in:
  • Chakroborty S  et al. Early presynaptic and postsynaptic calcium signaling abnormalities mask underlying synaptic depression in presymptomatic Alzheimer's disease mice. J Neurosci 32:8341-53 (2012). WB ; Mouse . Read more (PubMed: 22699914) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Whole heart lysate cleared by centrifuge)
Loading amount 30 µg
Specification Whole heart lysate cleared by centrifuge
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Odyssey blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
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Verified customer

Submitted Jun 08 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"