Overview

  • Product name
  • Description
    Rabbit polyclonal to KCNN2
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within the internal amino acids 72-121 (KNAAANVLRE TWLIYKNTKL VKKIDHAKVR KHQRKFLQAI HQLRSVKMEQ) of Human KCNN2 isoform b (NP_740721).

  • Positive control
    • Human fetal muscle lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85401 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images



  • Predicted band size : 26 kDa
    Western blot analysis of Human fetal muscle tissue lysate labeling KCNN2 with ab85401 at 1.0µg/ml.


  • Predicted band size : 26 kDa
    Western blot analysis of Human fetal lung tissue lysate labeling KCNN2 with ab85401 at 1.0µg/ml.


  • Predicted band size : 26 kDa
    Western blot analysis of Human fetal heart tissue lysate labeling KCNN2 with ab85401 at 1.0µg/ml.
  • Anti-KCNN2 antibody (ab85401) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 26 kDa
    12% gel
  • ab85401 staining KCNN2 in Rhesus macaque spinal cord by ICC/IF (Immunocytochemistry/immunofluorescence).  Samples were incubated with primary antibody (1/300). An Alexa Fluor®488-conjugated Donkey anti-rabbit (1/500) was used as the secondary antibody.

References

This product has been referenced in:
  • Willis M  et al. Small-conductance calcium-activated potassium type 2 channels (SK2, KCa2.2) in human brain. Brain Struct Funct 222:973-979 (2017). WB ; Human . Read more (PubMed: 27357310) »
  • Chakroborty S  et al. Early presynaptic and postsynaptic calcium signaling abnormalities mask underlying synaptic depression in presymptomatic Alzheimer's disease mice. J Neurosci 32:8341-53 (2012). WB ; Mouse . Read more (PubMed: 22699914) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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