The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 2 µg/ml.
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 70, 23 kDa (predicted molecular weight: 64, 26 kDa).Can be blocked with KCNN2 peptide (ab186680).
Forms a voltage-independent potassium channel activated by intracellular calcium. Activation is followed by membrane hyperpolarization. Thought to regulate neuronal excitability by contributing to the slow component of synaptic afterhyperpolarization. The channel is blocked by apamin.
Belongs to the potassium channel KCNN family. KCa2.2/KCNN2 subfamily.
ab99457 at 3.8 µg/ml staining KCNN2 in Human thyroid by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed, antibody was revealed by AP-staining.