Overview

  • Product nameAnti-KCNN2 antibody
    See all KCNN2 primary antibodies
  • Description
    Goat polyclonal to KCNN2
  • SpecificityRecognizes both Human isoforms (NP_067627.2 and NP_740721.1)
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Guinea pig, Cow, Dog, Orangutan
  • Immunogen

    Synthetic peptide: C-

    ESYDKHVTYNAER

    , corresponding to internal amino acids 546 - 557 of Human KCNN2 (NP_067627.2) and 197 - 209 of Human KCNN2 isoform b (NP_740721.1).

  • Positive control
    • Liver lysate Human thyroid

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab99457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 - 2 µg/ml.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 70, 23 kDa (predicted molecular weight: 64, 26 kDa).Can be blocked with KCNN2 peptide (ab186680).

Target

Anti-KCNN2 antibody images

  • Anti-KCNN2 antibody (ab99457) at 0.3 µg/ml + Human liver lysate, in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 64, 26 kDa
    Observed band size : 23,70 kDa (why is the actual band size different from the predicted?)
  • ab99457 at 3.8 µg/ml staining KCNN2 in Human thyroid by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed, antibody was revealed by AP-staining.

References for Anti-KCNN2 antibody (ab99457)

ab99457 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (spinal cord)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate, pH6
Permeabilization Yes - 0.1% Tween-20
Specification spinal cord
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Mrs. Teresa Garrett

Verified customer

Submitted Oct 28 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"