Anti-KCNN2/SK2 antibody (ab99457)
Key features and details
- Goat polyclonal to KCNN2/SK2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KCNN2/SK2 antibody
See all KCNN2/SK2 primary antibodies -
Description
Goat polyclonal to KCNN2/SK2 -
Host species
Goat -
Specificity
Recognizes both Human isoforms (NP_067627.2 and NP_740721.1) -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Guinea pig, Cow, Dog, Orangutan -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human liver lysate. IHC-P: Human thyroid tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab99457 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | (1) |
Use a concentration of 1 - 2 µg/ml.
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WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 70, 23 kDa (predicted molecular weight: 64, 26 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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IHC-P
Use a concentration of 1 - 2 µg/ml. |
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 70, 23 kDa (predicted molecular weight: 64, 26 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Forms a voltage-independent potassium channel activated by intracellular calcium. Activation is followed by membrane hyperpolarization. Thought to regulate neuronal excitability by contributing to the slow component of synaptic afterhyperpolarization. The channel is blocked by apamin. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the potassium channel KCNN family. KCa2.2/KCNN2 subfamily. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 404177 Cow
- Entrez Gene: 474640 Dog
- Entrez Gene: 3781 Human
- Entrez Gene: 140492 Mouse
- Entrez Gene: 100172000 Orangutan
- Entrez Gene: 54262 Rat
- Omim: 605879 Human
- SwissProt: Q6PJI0 Human
see all -
Alternative names
- Apamin sensitive small conductance Ca2+ activated potassium antibody
- Apamin sensitive small conductance Ca2+ activated potassium channel antibody
- hSK2 antibody
see all
Images
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Anti-KCNN2/SK2 antibody (ab99457) at 0.3 µg/ml + Human liver lysate, in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 64, 26 kDa
Observed band size: 23,70 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab99457 has been referenced in 1 publication.
- Du H et al. The Cation Channel TMEM63B Is an Osmosensor Required for Hearing. Cell Rep 31:107596 (2020). PubMed: 32375046