Overview

  • Product name
  • Description
    Rabbit polyclonal to KCNN3
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide derived from residues 647 - 696 of human KCNN3.

    .

  • Positive control
    • Daudi cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab28631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/62500.
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 82 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Forms a voltage-independent potassium channel activated by intracellular calcium. Activation is followed by membrane hyperpolarization. Thought to regulate neuronal excitability by contributing to the slow component of synaptic afterhyperpolarization. The channel is blocked by apamin.
  • Sequence similarities
    Belongs to the potassium channel KCNN family. KCa2.3/KCNN3 subfamily.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • hSK3 antibody
    • KCa2.3 antibody
    • Kcnn3 antibody
    • KCNN3_HUMAN antibody
    • Potassium calcium activated channel subfamily N member 3 antibody
    • Potassium channel calcium activated intermediate/small conductance subfamily N alpha member 3 antibody
    • Potassium channel, calcium activated, intermediate/small conductance, subfamily N, member 3 antibody
    • Potassium intermediate/small conductance calcium activated channel subfamily N member 3 antibody
    • SK3 antibody
    • SKCa 3 antibody
    • SKCa3 antibody
    • Small conductance calcium-activated potassium channel protein 3 antibody
    see all

Anti-KCNN3 antibody images

  • Sample Type : Rhesus macaque spinal cord. Primary Antibody Dilution : 1:300. Secondary Antibody : Donkey anti Rabbit 488. Secondary Antibody Dilution : 1:500. Color/Signal Descriptions : Green: KCNN3. Gene Name : KCNN3.
  • Anti-KCNN3 antibody (ab28631) at 2 µg/ml + Daudi (Human Burkitt’s lymphoma cell line) Whole Cell Lysate 10ug.

    Secondary
    HRP conjugated anti-Rabbit IgG

    Predicted band size : 82 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)


  • Predicted band size : 82 kDa
    ab28631 at 2µg/ml used in Western blot. Negative control: mouse kidney lysate, positive sample: mouse whole brain lysate. The secondary antibody was goat anti-rabbit and used at 1/10,000 dilution.

References for Anti-KCNN3 antibody (ab28631)

ab28631 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Denaturing (8% gel)
Sample
Pig Cell lysate - whole cell (Endothelial Cell)
Specification
Endothelial Cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Feb 27 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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