Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab28631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/62500.
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 82 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-KCNN3 antibody images

  • Sample Type : Rhesus macaque spinal cord. Primary Antibody Dilution : 1:300. Secondary Antibody : Donkey anti Rabbit 488. Secondary Antibody Dilution : 1:500. Color/Signal Descriptions : Green: KCNN3. Gene Name : KCNN3.
  • Anti-KCNN3 antibody (ab28631) at 2 µg/ml + Daudi (Human Burkitt’s lymphoma cell line) Whole Cell Lysate 10ug.

    Secondary
    HRP conjugated anti-Rabbit IgG

    Predicted band size : 82 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)


  • Predicted band size : 82 kDa
    ab28631 at 2µg/ml used in Western blot. Negative control: mouse kidney lysate, positive sample: mouse whole brain lysate. The secondary antibody was goat anti-rabbit and used at 1/10,000 dilution.

References for Anti-KCNN3 antibody (ab28631)

ab28631 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Non-reduced Denaturing (8% gel)
Sample Pig Cell lysate - whole cell (Endothelial Cell)
Specification Endothelial Cell
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Feb 27 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"