Anti-KCNN4 antibody (ab215990)
Key features and details
- Rabbit polyclonal to KCNN4
- Suitable for: IHC-P, WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-KCNN4 antibody
See all KCNN4 primary antibodies -
Description
Rabbit polyclonal to KCNN4 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Human -
Immunogen
Synthetic peptide within Human KCNN4 aa 300-400 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O15554 -
Positive control
- Rat brain tissue; rat brain and rat heart lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X
Aqueous buffered solution -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab215990 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (1) |
1/100 - 1/1000. Predicted molecular weight: 48 kDa.
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Notes |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/100 - 1/1000. Predicted molecular weight: 48 kDa. |
Target
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Function
Forms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T cells. The channel is blocked by clotrimazole and charybdotoxin but is insensitive to apamin. -
Tissue specificity
Widely expressed in non-excitable tissues. -
Sequence similarities
Belongs to the potassium channel KCNN family. KCa3.1/KCNN4 subfamily. -
Post-translational
modificationsPhosphorylation at His-358 by NDKB activates the channel, and conversely it's dephosphorylation by PHPT1 inhibits the channel. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3783 Human
- Entrez Gene: 16534 Mouse
- Entrez Gene: 65206 Rat
- Omim: 602754 Human
- SwissProt: O15554 Human
- SwissProt: O89109 Mouse
- SwissProt: Q9QYW1 Rat
- Unigene: 10082 Human
see all -
Alternative names
- DHS2 antibody
- hIKCa1 antibody
- hKCa4 antibody
see all
Images
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All lanes : Anti-KCNN4 antibody (ab215990) at 1/200 dilution
Lane 1 : rat brain lysate
Lane 2 : rat heart lysate
Secondary
All lanes : HRP-conjugated secondary antibody at 1/3000 dilution
Predicted band size: 48 kDa
Observed band size: 45, 50 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat brain tissue labeling KCNN4 with ab215990 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab215990 has been referenced in 2 publications.
- Yang M et al. Adipose-derived stem cells overexpressing SK4 calcium-activated potassium channel generate biological pacemakers. Int J Mol Med 44:2103-2112 (2019). PubMed: 31638180
- Zhao H et al. Overexpression of the medium-conductance calcium-activated potassium channel (SK4) and the HCN2 channel to generate a biological pacemaker. Mol Med Rep 20:3406-3414 (2019). PubMed: 31432175