Anti-KCNN4 antibody (ab75956)

Overview

  • Product nameAnti-KCNN4 antibody
    See all KCNN4 primary antibodies
  • Description
    Rabbit polyclonal to KCNN4
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human KCNN4.

    (Peptide available as ab87266.)

  • Positive control
    • This antibody gave a positive signal in the following human tissue lysates: Skin Placenta

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab75956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).

Target

  • FunctionForms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T cells. The channel is blocked by clotrimazole and charybdotoxin but is insensitive to apamin.
  • Tissue specificityWidely expressed in non-excitable tissues.
  • Sequence similaritiesBelongs to the potassium channel KCNN family. KCa3.1/KCNN4 subfamily.
  • Post-translational
    modifications
    Phosphorylation at His-358 by NDKB activates the channel, and conversely it's dephosphorylation by PHPT1 inhibits the channel.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • hIKCa1 antibody
    • hKCa4 antibody
    • hSK4 antibody
    • IK1 antibody
    • IKCa1 antibody
    • Intermediate conductance calcium activated potassium channel protein 4 antibody
    • Intermediate conductance calcium-activated potassium channel protein 4 antibody
    • KCa3.1 antibody
    • KCa4 antibody
    • KCNN 4 antibody
    • Kcnn4 antibody
    • KCNN4_HUMAN antibody
    • Potassium intermediate/small conductance calcium activated channel, subfamily N, member 4 antibody
    • Putative Gardos channel antibody
    • SK4 antibody
    • SKCa 4 antibody
    • SKCa4 antibody
    see all

Anti-KCNN4 antibody images

  • All lanes : Anti-KCNN4 antibody (ab75956) at 1 µg/ml

    Lane 1 : Human skin tissue lysate - total protein (ab30166)
    Lane 2 : Human placenta tissue lysate - total protein (ab29745)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References for Anti-KCNN4 antibody (ab75956)

ab75956 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your patience. Here is the homology between human and mouse for the following antibodies (based on the alignment of their immunogen sequences) ab55452: homology = 94% - crossreactivity very likely, but not tested ab57662: homolog...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Pig Tissue lysate - whole (Right Coronary Artery & Lung)
Loading amount 20 µg
Specification Right Coronary Artery & Lung
Gel Running Conditions Non-reduced Non-Denaturing (Native) (4-20% Tris Ready Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Aug 02 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"