Fusion protein: AAASSPPRAE RKRWGWGRLP GARRGSAGLA KKCPFSLELA EGGPAGGALY APIAPGAPGP APPASPAAPA APPVASDLGP RPPVSLDPRV SIYSTRRPVL, corresponding to amino acids 2-101 of Human KCNQ1 (accession number: P51787)
Our Abpromise guarantee covers the use of ab84819 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|IHC-P||Use a concentration of 0.1 - 1 µg/ml.|
|IHC-Fr||Use a concentration of 0.1 - 1 µg/ml.|
|WB||Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 75 kDa.|
|ICC/IF||Use a concentration of 1 - 10 µg/ml.|
ab84819 staining KCNQ1 in human hippocampal tissue by IHC-P (Bouin's fixative fixed paraffin embedded). The sample was incubated with primary antibody at 100 and with a fluorophore conjugated anti mouse secondary at 1/50 dilution.