Overview

  • Product name
  • Description
    Goat polyclonal to KCNQ4
  • Specificity
    ab82096 is expected to recognise both reported isoforms (NP_004691.2; NP_751895.1).
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    C-DKGPSDAEVVDE

    , corresponding to internal sequence amino acids 601-612 of Human KCNQ4 (NP_004691.2).

  • Positive control
    • Human Brain (Cerebellum) lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab82096 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Antibody detection limit dilution 1/4000.
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 77 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Probably important in the regulation of neuronal excitability. May underlie a potassium current involved in regulating the excitability of sensory cells of the cochlea. KCNQ4 channels are blocked by linopirdin, XE991 and bepridil, whereas clofilium is without significant effect. Muscarinic agonist oxotremorine-M strongly suppress KCNQ4 current in CHO cells in which cloned KCNQ4 channels were coexpressed with M1 muscarinnic receptors.
    • Tissue specificity
      Expressed in the outer, but not the inner, sensory hair cells of the cochlea. Slightly expressed in heart, brain and skeletal muscle.
    • Involvement in disease
      Defects in KCNQ4 are the cause of deafness autosomal dominant type 2A (DFNA2A) [MIM:600101]. DFNA2A is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
    • Sequence similarities
      Belongs to the potassium channel family. KQT (TC 1.A.1.15) subfamily. Kv7.4/KCNQ4 sub-subfamily.
    • Domain
      The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
      The A-domain tail carries the major determinants of channel assembly specificity. Its coiled-coil region is Four-stranded.
    • Cellular localization
      Basal cell membrane. Situated at the basal membrane of cochlear outer hair cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • DFNA 2 antibody
      • DFNA2 antibody
      • KCNQ 4 antibody
      • Kcnq4 antibody
      • KCNQ4_HUMAN antibody
      • KQT like 4 antibody
      • KQT-like 4 antibody
      • KV7.4 antibody
      • Potassium channel KQT like 4 antibody
      • Potassium channel subunit alpha KvLQT4 antibody
      • Potassium voltage gated channel KQT like protein 4 antibody
      • Potassium voltage gated channel KQT like subfamily member 4 antibody
      • Potassium voltage gated channel subfamily KQT member 4 antibody
      • Potassium voltage-gated channel subfamily KQT member 4 antibody
      • Voltage gated potassium channel subunit Kv7.4 antibody
      • Voltage-gated potassium channel subunit Kv7.4 antibody
      see all

    Images

    • Anti-KCNQ4 antibody (ab82096) at 2 µg/ml + Human Cerebellum lysate (in RIPA buffer). at 35 µg
      Developed using the ECL technique

      Predicted band size : 77 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)

    References

    ab82096 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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