Anti-KCNQ4 antibody [N43/6] (ab84820)
Key features and details
- Mouse monoclonal [N43/6] to KCNQ4
- Suitable for: IHC-Fr, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-KCNQ4 antibody [N43/6] -
Description
Mouse monoclonal [N43/6] to KCNQ4 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Fr, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chimpanzee -
Immunogen
Fusion protein corresponding to Human KCNQ4 aa 1-100.
Database link: P56696 -
Positive control
- Lysate from transfected COS-1 cells transiently expressing KCNQ4.
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General notes
The clone number has been updated from S43-6 to N43/6, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N43/6 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
1/1000.
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IHC-P |
1/1000.
Fixation: Bouin’s Fixative |
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WB |
1/1000. Predicted molecular weight: 77 kDa.
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Notes |
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IHC-Fr
1/1000. |
IHC-P
1/1000. Fixation: Bouin’s Fixative |
WB
1/1000. Predicted molecular weight: 77 kDa. |
Target
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Function
Probably important in the regulation of neuronal excitability. May underlie a potassium current involved in regulating the excitability of sensory cells of the cochlea. KCNQ4 channels are blocked by linopirdin, XE991 and bepridil, whereas clofilium is without significant effect. Muscarinic agonist oxotremorine-M strongly suppress KCNQ4 current in CHO cells in which cloned KCNQ4 channels were coexpressed with M1 muscarinnic receptors. -
Tissue specificity
Expressed in the outer, but not the inner, sensory hair cells of the cochlea. Slightly expressed in heart, brain and skeletal muscle. -
Involvement in disease
Defects in KCNQ4 are the cause of deafness autosomal dominant type 2A (DFNA2A) [MIM:600101]. DFNA2A is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. -
Sequence similarities
Belongs to the potassium channel family. KQT (TC 1.A.1.15) subfamily. Kv7.4/KCNQ4 sub-subfamily. -
Domain
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
The A-domain tail carries the major determinants of channel assembly specificity. Its coiled-coil region is Four-stranded. -
Cellular localization
Basal cell membrane. Situated at the basal membrane of cochlear outer hair cells. - Information by UniProt
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Database links
- Entrez Gene: 456800 Chimpanzee
- Entrez Gene: 9132 Human
- Entrez Gene: 60613 Mouse
- Entrez Gene: 298496 Rat
- Omim: 603537 Human
- SwissProt: P56696 Human
- SwissProt: Q9JK97 Mouse
- SwissProt: Q9JK96 Rat
see all -
Alternative names
- DFNA 2 antibody
- DFNA2 antibody
- KCNQ 4 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded human hippocampus tissue labelling KCNQ4 with ab84820 at 1/1000 dilution, for 1 hour at RT followed by secondary antibody Goat Anti-Mouse (FITC) (Green) at 1/50 dilution, for 1 hour at RT.
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Immunohistochemical analysis of Mouse brain tissue sections labeling KCNQ4 with ab84820 at 1/1000 dilution for 1 hour at room temperature.
Secondary Antibody: HRP/DAB Detection
System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at room temperature.
Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at room temperature
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All lanes : Anti-KCNQ4 antibody [N43/6] (ab84820) at 1/1000 dilution
Lane 1 : Molecular weight marker
Lane 2 : Mixed cell lysates prepared from rat tissues Brain, Heart, Kidney, Liver, Lung, Pancreas, Skeletal Muscle, Spleen, Testes, Thymus, and H9C2 cells at 15 µg
Secondary
Lane 2 : Sheep Anti-Mouse IgG: HRP
Predicted band size: 77 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab84820 has been referenced in 4 publications.
- Wang Q et al. miR-153/KCNQ4 axis contributes to noise-induced hearing loss in a mouse model. J Physiol Sci 71:28 (2021). PubMed: 34479475
- Xiong H et al. Inhibition of Histone Methyltransferase G9a Attenuates Noise-Induced Cochlear Synaptopathy and Hearing Loss. J Assoc Res Otolaryngol N/A:N/A (2019). PubMed: 30710318
- Brueggemann LI et al. Differential protein kinase C-dependent modulation of Kv7.4 and Kv7.5 subunits of vascular Kv7 channels. J Biol Chem 289:2099-111 (2014). WB, ICC/IF . PubMed: 24297175
- Udagawa T et al. Inwardly rectifying potassium channel Kir4.1 is localized at the calyx endings of vestibular afferents. Neuroscience 215:209-16 (2012). PubMed: 22546335