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Fusion protein: AEAPPRRLGL GPPPGDAPRA ELVALTAVQS EQGEAGGGGS PRRLGLLGSP LPPGAPLPGP GSGSGSACGQ RSSAAH, corresponding to amino acids 2-77 of Human KCNQ4 (accession number P56696).
Our Abpromise guarantee covers the use of ab84820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 10 mg/ml. Predicted molecular weight: 77 kDa.|
|ICC/IF||Use a concentration of 1 - 10 µg/ml.|
|IP||Use at an assay dependent dilution.|
ab84820 staining KCNQ4 in KCNQ4 transfected NIH3T3 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA, permeabilized with 0.5% Triton X-100 in PBS and blocked with 4% Milk in PBS for 60 minutes at 21°C. Samples were incubated with primary antibody (1/1000 in PBS) for 1 hour at 21°C. An Alexa Fluor®594-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.
ab84820 staining KCNQ4 in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections).