Overview

  • Product nameAnti-KCNQ4 antibody [S43-6]
    See all KCNQ4 primary antibodies
  • Description
    Mouse monoclonal [S43-6] to KCNQ4
  • Tested applicationsSuitable for: WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Fusion protein: AEAPPRRLGL GPPPGDAPRA ELVALTAVQS EQGEAGGGGS PRRLGLLGSP LPPGAPLPGP GSGSGSACGQ RSSAAH, corresponding to amino acids 2-77 of Human KCNQ4 (accession number P56696).

  • Positive control
    • Lysate from transfected COS-1 cells transiently expressing KCNQ4.

Properties

Applications

Our Abpromise guarantee covers the use of ab84820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 10 mg/ml. Predicted molecular weight: 77 kDa.
ICC/IF Use a concentration of 1 - 10 µg/ml.
IP Use at an assay dependent dilution.

Target

  • FunctionProbably important in the regulation of neuronal excitability. May underlie a potassium current involved in regulating the excitability of sensory cells of the cochlea. KCNQ4 channels are blocked by linopirdin, XE991 and bepridil, whereas clofilium is without significant effect. Muscarinic agonist oxotremorine-M strongly suppress KCNQ4 current in CHO cells in which cloned KCNQ4 channels were coexpressed with M1 muscarinnic receptors.
  • Tissue specificityExpressed in the outer, but not the inner, sensory hair cells of the cochlea. Slightly expressed in heart, brain and skeletal muscle.
  • Involvement in diseaseDefects in KCNQ4 are the cause of deafness autosomal dominant type 2A (DFNA2A) [MIM:600101]. DFNA2A is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
  • Sequence similaritiesBelongs to the potassium channel family. KQT (TC 1.A.1.15) subfamily. Kv7.4/KCNQ4 sub-subfamily.
  • DomainThe segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
    The A-domain tail carries the major determinants of channel assembly specificity. Its coiled-coil region is Four-stranded.
  • Cellular localizationBasal cell membrane. Situated at the basal membrane of cochlear outer hair cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • DFNA 2 antibody
    • DFNA2 antibody
    • KCNQ 4 antibody
    • Kcnq4 antibody
    • KCNQ4_HUMAN antibody
    • KQT like 4 antibody
    • KQT-like 4 antibody
    • KV7.4 antibody
    • Potassium channel KQT like 4 antibody
    • Potassium channel subunit alpha KvLQT4 antibody
    • Potassium voltage gated channel KQT like protein 4 antibody
    • Potassium voltage gated channel KQT like subfamily member 4 antibody
    • Potassium voltage gated channel subfamily KQT member 4 antibody
    • Potassium voltage-gated channel subfamily KQT member 4 antibody
    • Voltage gated potassium channel subunit Kv7.4 antibody
    • Voltage-gated potassium channel subunit Kv7.4 antibody
    see all

Anti-KCNQ4 antibody [S43-6] images

  • ab84820 staining KCNQ4 in KCNQ4 transfected NIH3T3 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA, permeabilized with 0.5% Triton X-100 in PBS and blocked with 4% Milk in PBS for 60 minutes at 21°C. Samples were incubated with primary antibody (1/1000 in PBS) for 1 hour at 21°C. An Alexa Fluor®594-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ab84820 staining KCNQ4 in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections).

  • All lanes : Anti-KCNQ4 antibody [S43-6] (ab84820)

    Lane 1 : Molecular weight marker
    Lane 2 : Mixed cell lysates prepared from rat tissues Brain, Heart, Kidney, Liver, Lung, Pancreas, Skeletal Muscle, Spleen, Testes, Thymus, and H9C2 cells at 10 µg


    Predicted band size : 77 kDa
  • ab84820 staining KCNQ4 in human hippocampal tissue by IHC-P (Bouin's fixed paraffin embedded).

References for Anti-KCNQ4 antibody [S43-6] (ab84820)

This product has been referenced in:
  • Brueggemann LI  et al. Differential protein kinase C-dependent modulation of Kv7.4 and Kv7.5 subunits of vascular Kv7 channels. J Biol Chem 289:2099-111 (2014). WB, ICC/IF . Read more (PubMed: 24297175) »
  • Udagawa T  et al. Inwardly rectifying potassium channel Kir4.1 is localized at the calyx endings of vestibular afferents. Neuroscience 215:209-16 (2012). Read more (PubMed: 22546335) »

See all 3 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C
Sample Mouse Cell (NIH3T3 TRANSFECTED KCNQ4 CELL)
Specification NIH3T3 TRANSFECTED KCNQ4 CELL
Permeabilization Yes - triton X100 0.5%
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 31 2014

Thank you for your answer and for your cooperation.

I am sorry that the ab84820 required more optimisation than expected and for the inconvenience this has caused.As requested, I agreed to send a free alternative antibody in exchange for the ...

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Thank you for your reply.

We can send you a new antibody in exchange for your feedback, however I am not sure whether this will resolve your question.

I have discussed this case also with a colleague of mine. Indeed, we both think t...

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I just write you to inquire whether you have yet had the opporunity to look into my comments/suggestions? Please let me know also if you have any questions or concerns - I would be happy to discuss them with you. If the problem has been solved, I would...

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Thank you for your reply.

I am sorry for the confusion of the origin of the samples. Indeed, as you are using human samples, there are two isoformes described. One is at 71kD and the other one is at 77kD. Please see also this link to SwissPro...

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Thank you for taking time to complete our questionnaire. I am sorry that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitor...

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Thank you for calling us.

As we discussed on the phone, I have contacted thelaboratory for the additional information. I am also attaching the discussed questionnaire so that we can gather further information regarding the samples tested and ...

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Thank you very much for your email.

I have now received an email from lab. What my colleague has suggested is attached below;

Multiple bands are very common with ion channels in general. Proteolysis is very common – it has to...

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I can confirm that I have send an email to my colleaguein lab and requested the productspecific WB protocol; I will be able to forward the same once I get a reply from them.

Many thanks for having patience.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"