Anti-KCNT1/SLACK antibody [N3/26] (ab94578)
Key features and details
- Mouse monoclonal [N3/26] to KCNT1/SLACK
- Suitable for: ICC/IF, IHC-Fr, Flow Cyt, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-KCNT1/SLACK antibody [N3/26]
See all KCNT1/SLACK primary antibodies -
Description
Mouse monoclonal [N3/26] to KCNT1/SLACK -
Host species
Mouse -
Specificity
Detects ~140kDa. Weak human detection. Does not cross-react with KCNT2/Slo2.1/Slick.
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Tested applications
Suitable for: ICC/IF, IHC-Fr, Flow Cyt, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Rat KCNT1/SLACK aa 1150 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9Z258 -
Positive control
- Flow Cyt: SH-SY5Y cells. WB: Rat brain lysate.
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General notes
The clone number has been updated from S3-26 to N3/26, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N3/26 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab94578 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB | (1) |
1/1000. Predicted molecular weight: 137 kDa.
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IHC-P |
1/1000.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
1/1000. Predicted molecular weight: 137 kDa. |
IHC-P
1/1000. |
Target
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Function
Outwardly rectifying potassium channel subunit that may co-assemble with other Slo-type channel subunits. Activated by high intracellular sodium or chloride levels. Activated upon stimulation of G-protein coupled receptors, such as CHRM1 and GRIA1. May be regulated by calcium in the absence of sodium ions (in vitro). -
Tissue specificity
Highest expression in liver, brain and spinal cord. Lowest expression in skeletal muscle. -
Sequence similarities
Belongs to the potassium channel family. Calcium-activated (TC 1.A.1.3) subfamily. KCa4.1/KCNT1 sub-subfamily.
Contains 1 RCK N-terminal domain. -
Post-translational
modificationsPhosphorylated by protein kinase C. Phosphorylation of the C-terminal domain increases channel activity. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 57582 Human
- Entrez Gene: 227632 Mouse
- Entrez Gene: 60444 Rat
- Omim: 608167 Human
- SwissProt: Q5JUK3 Human
- SwissProt: Q6ZPR4 Mouse
- SwissProt: Q9Z258 Rat
- Unigene: 104950 Human
see all -
Alternative names
- bA100C15.2 antibody
- EIEE14 antibody
- ENFL5 antibody
see all
Images
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Anti-KCNT1/SLACK antibody [N3/26] (ab94578) at 1/1000 dilution + Rat brain lysate at 15 µg
Secondary
HRP-conjugated sheet anti-mouse IgG
Predicted band size: 137 kDaMembrane was blocked with 1.5% BSA for 30 minutes at room temperature. Primari antibody was incubated for 2 hours at room temperature.
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Overlay histogram showing SH-SY5Y cells stained with ab94578 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab94578, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab94578 has been referenced in 2 publications.
- Li Y et al. A Role for Transmembrane Protein 16C/Slack Impairment in Excitatory Nociceptive Synaptic Plasticity in the Pathogenesis of Remifentanil-induced Hyperalgesia in Rats. Neurosci Bull 37:669-683 (2021). PubMed: 33779892
- Shore AN et al. Reduced GABAergic Neuron Excitability, Altered Synaptic Connectivity, and Seizures in a KCNT1 Gain-of-Function Mouse Model of Childhood Epilepsy. Cell Rep 33:108303 (2020). PubMed: 33113364