Overview

  • Product name
    Anti-KCNU1 antibody [N2/16]
  • Description
    Mouse monoclonal [N2/16] to KCNU1
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Fusion protein: DSSPSIQAQN NSTNATTPLA QGSNFFDSHH ADESHDLYPV DDTGERWSQH HHSRVYPLDT LDASDIVQEK, corresponding to amino acids 1052-1121 of Mouse KCNU1 (AAB99742).

  • Positive control
    • Mouse brain lysate
  • General notes

    The clone number has been updated from S2-16 to N2/16, both clone numbers name the same antibody clone.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab94586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
IHC-Fr
ICC/IF
  • Application notes
    ICC/IF: Use at a concentration of 0.1-1.0µg/mL (perox) or 1.0-10µg/mL (IF).
    IHC-P: Use at a concentration of 0.1-1.0µg/mL (perox) or 1.0-10µg/mL (IF).
    IHC-Fr: Use at a concentration of 0.1-1.0µg/mL (perox) or 1.0-10µg/mL (IF).
    WB: Use at a concentration of 1 - 10 µg/ml. Predicted molecular weight: 130 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Testis-specific potassium channel activated by both intracellular pH and membrane voltage that mediates export of K(+). May represent the primary spermatozoan K(+) current. In contrast to KCNMA1/SLO1, it is not activated by Ca(2+) or Mg(2+). Critical for fertility. May play an important role in sperm osmoregulation required for the acquisition of normal morphology and motility when faced with osmotic challenges, such as those experienced after mixing with seminal fluid and entry into the vagina.
    • Tissue specificity
      Testis-specific.
    • Sequence similarities
      Belongs to the potassium channel family. Calcium-activated (TC 1.A.1.3) subfamily. KCa5.1/KCNU1 sub-subfamily.
      Contains 1 RCK N-terminal domain.
    • Domain
      The S4 segment, which is characterized by a series of positively charged amino acids at every third position, is part of the voltage-sensor.
      The pore-forming domain (also referred as P region) is imbedded into the membrane, and forms the selectivity filter of the pore. It contains the signature sequence of potassium channels that displays selectivity to potassium.
      The RCK N-terminal domain mediates the homotetramerization, thereby promoting the assembly of monomers into functional potassium channel.
      The C-terminal cytosolic region confers the pH-dependence.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • subfamily M subunit alpha-3 antibody
      • Calcium activated potassium channel subfamily M subunit alpha 3 antibody
      • Calcium activated potassium channel subunit alpha 3 antibody
      • Calcium-activated potassium channel antibody
      • Calcium-activated potassium channel subunit alpha-3 antibody
      • KCa5 antibody
      • KCa5.1 antibody
      • Kcnma3 antibody
      • KCNMC1 antibody
      • KCNU 1 antibody
      • KCNU1 antibody
      • KCNU1_HUMAN antibody
      • Potassium channel subfamily U member 1 antibody
      • Slo3 antibody
      • Slowpoke homolog 3 antibody
      see all

    Images



    • Predicted band size : 130 kDa
      Western blot detection of KCNU1 in Rat brain membrane lysates using ab94586 at 1:1000 dilution.

    References

    ab94586 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab94586.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up