The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa). Primary incubation time: 1 hour.
The KCTD11 gene encodes a protein that has been identified as a suppressor of Hedgehog signaling. Its inactivation might lead to a deregulation of the tumor promoting Hedgehog pathway in medulloblastoma.
Plays a role as a marker and a regulator of neuronal differentiation; Up-regulated by a variety of neurogenic signals, such as retinoic acid, epidermal growth factor/EGF and NGFB/nerve growth factor. Induces apoptosis, growth arrest and the expression of cyclin-dependent kinase inhibitor CDKN1B. Plays a role as a tumor repressor and inhibits cell growth and tumorigenicity of medulloblastoma (MDB). Functions as antagonist of the Hedgehog pathway on cell proliferation and differentiation by affecting the nuclear transfer of transcrition factor GLI1, thus maintaining cerebellar granule cells in undifferentiated state.
Retinoic acid EGF NGF induced gene protein antibody
Western blot - KCTD11 antibody (ab113423)
Lane 1 : Anti-KCTD11 antibody (ab113423) at 1 µg/ml Lane 2 : Anti-KCTD11 antibody (ab113423) at 1 mg/ml Lane 3 : anti-DYKDDDDK Tag at 1/5000 dilution
Lane 1 : HEK293 lysate, (in RIPA buffer) overexpressing Human KCTD11 with DYKDDDDK tag at 10 µg Lane 2 : HEK293 lysate, Mock-transfected Lane 3 : HEK293 lysate, (in RIPA buffer) overexpressing Human KCTD11 with DYKDDDDK tag at 10 µg