Overview

  • Product nameAnti-KCTD18 antibody
    See all KCTD18 primary antibodies
  • Description
    Rabbit polyclonal to KCTD18
  • Specificityab33709 recognises KCTD18.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    A region within synthetic peptide: YLNTSGASCE SRIIGVYATK TDGTDAIEKQ LGGRIHSKGI FKREAGNNVQ, corresponding to internal sequence amino acids 147-196 of Human KCTD18

  • Positive control
    • Jurkat cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab33709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/312500.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-KCTD18 antibody images

  • Anti-KCTD18 antibody (ab33709) at 5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti Rabbit IgG at a dilution of 1/50000 - 1/100000

    Predicted band size : 47 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)

References for Anti-KCTD18 antibody (ab33709)

ab33709 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (THP-1)
Loading amount 20 µg
Specification THP-1
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Dec 10 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"