Overview

  • Product name
    Anti-KCTD20 antibody
  • Description
    Rabbit polyclonal to KCTD20
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    antigen sequence: LAEDIKGSCF QSGNKRNHEP FIAPERFGNS SVGFGSNSHS QAPEKVTLLV DGTRFVVNPQ IFTAHPDTML, corresponding to amino acids 74-143 of Human KCTD20.

  • Positive control
    • Human anal skin tissue; RT-4 and U-251 MG cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122094 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

Anti-KCTD20 antibody images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in endoplasmic reticulum. Recommended concentration of ab122094 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
    Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
  • ab122094, at 1/35 dilution, staining KCTD20 in paraffin-embedded Human anal skin tissue by Immunohistochemistry.
  • All lanes : Anti-KCTD20 antibody (ab122094) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

References for Anti-KCTD20 antibody (ab122094)

ab122094 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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