Recombinant Anti-KCTD21 antibody [EPR12873(2)] (ab192259)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12873(2)] to KCTD21
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-KCTD21 antibody [EPR12873(2)]
See all KCTD21 primary antibodies -
Description
Rabbit monoclonal [EPR12873(2)] to KCTD21 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Human fetal brain lysate. Mouse and rat brain lysate. Caco-2, C6, RAW264.7, PC-12 and NIH/3T3 cell lysate. Caco-2 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS, 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12873(2) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/220.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000. Predicted molecular weight: 30 kDa.
|
Notes |
---|
Flow Cyt (Intra)
1/220. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000. Predicted molecular weight: 30 kDa. |
Target
-
Relevance
KCTD21 contains 1 BTB (POZ) domain. The exact function of KCTD21 remains unknown. -
Database links
- Entrez Gene: 283219 Human
- Entrez Gene: 622320 Mouse
- Entrez Gene: 499209 Rat
- SwissProt: Q4G0X4 Human
- SwissProt: Q3URF8 Mouse
-
Alternative names
- BTB/POZ domain containing protein KCTD21 antibody
- potassium channel tetramerisation domain containing 21 antibody
Images
-
Anti-KCTD21 antibody [EPR12873(2)] (ab192259) at 1/1000 dilution + human fetal brain lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 30 kDa -
Anti-KCTD21 antibody [EPR12873(2)] (ab192259) at 1/1000 dilution + Caco-2 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 30 kDa -
All lanes : Anti-KCTD21 antibody [EPR12873(2)] (ab192259) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : C6 cell lysate
Lane 4 : RAW264.7 cell lysate
Lane 5 : PC-12 cell lysate
Lane 6 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 30 kDa -
Intracellular Flow Cytometry analysis of Caco-2 (Human colorectal adenocarcinoma cell line) cells labeling KCTD21 using ab192259 at a 1/220 dilution (pink). Goat anti-rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (2)
ab192259 has been referenced in 2 publications.
- Angrisani A et al. Specific Protein 1 and p53 Interplay Modulates the Expression of the KCTD-Containing Cullin3 Adaptor Suppressor of Hedgehog 2. Front Cell Dev Biol 9:638508 (2021). PubMed: 33898425
- Spiombi E et al. KCTD15 inhibits the Hedgehog pathway in Medulloblastoma cells by increasing protein levels of the oncosuppressor KCASH2. Oncogenesis 8:64 (2019). PubMed: 31685809