The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 30 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceKCTD4 contains a potassium channel tetramerisation domain. The N terminal, cytoplasmic tetramerisation domain (T1) of voltage-gated potassium channels encodes molecular determinants for subfamily specific assembly of alpha subunits into functional tetrameric channels. The specific function of KCTD4 is unknown.