Anti-KDEL antibody [10C3] (Phycoerythrin) (ab115636)

Overview

  • Product nameAnti-KDEL antibody [10C3] (Phycoerythrin)
    See all KDEL primary antibodies
  • Description
    Mouse monoclonal [10C3] to KDEL (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Specificityab115636 cross reacts with proteins containing the KDEL sequence.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity

    Predicted to work with: Bird, Plants, all Mammals
  • Immunogen

    Synthetic peptide:

    SEKDEL

    , corresponding to amino acids 649-654 of Rat KDEL.

  • Positive control
    • Jurkat cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number10C3
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab115636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100.

Target

  • RelevanceThe sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins. 78 and 94 kDa glucose regulated proteins (GRP 78) and GRP 94 respectively and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.
  • Cellular localizationEndoplasmic reticulum
  • Alternative names
    • KDEL antibody
    • Lys Asp Glu Leu antibody

Anti-KDEL antibody [10C3] (Phycoerythrin) images

  • ab115636 at 10µg/ml staining KDEL in 106 Jurkat cells by Flow cytometry (shown in red).
    Grey: unstained;Yellow: isotype control.

References for Anti-KDEL antibody [10C3] (Phycoerythrin) (ab115636)

ab115636 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"