Overview

  • Product name
    Anti-KDEL antibody [MAC 256]
    See all KDEL primary antibodies
  • Description
    Rat monoclonal [MAC 256] to KDEL
  • Specificity
    This monoclonal antibody reacts with the carboxy-terminal KDEL motif responsible for the endoplasmic reticulum (ER) localisation of several proteins. It is applicable as an ER marker in a wide variety of species (insects, mammals, plants) and also marks the ER-derived ‘ricinosome’ compartment in senescing plant tissue. It reacts strongly with protein
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with a wide range of species.
  • Immunogen

    Auxin binding protein (Maize) containing KDEL motif

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    MAC 256
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/300. PubMed: 20392701
WB Use at an assay dependent dilution.

Target

  • Relevance
    The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins. 78 and 94 kDa glucose regulated proteins (GRP 78) and GRP 94 respectively and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.
  • Cellular localization
    Endoplasmic reticulum
  • Alternative names
    • KDEL antibody
    • Lys Asp Glu Leu antibody

Anti-KDEL antibody [MAC 256] images

  • ab50601 at 1/300 dilution staining KDEL-ER Marker in Chinese Hamster Ovary cells by Immunocytochemistry/ Immunofluorescence. Cells were seeded in 24-well plates on glass coverslips coated with 25 μg/μl fibronectin. An ALexa Fluor labelled anti rat secondary was used at 5µg/ml.

References for Anti-KDEL antibody [MAC 256] (ab50601)

This product has been referenced in:
  • Tauscher PM  et al. Adaptive protein divergence of BMP ligands takes place under developmental and evolutionary constraints. Development 143:3742-3750 (2016). Read more (PubMed: 27578781) »
  • Huang Y  et al. Acsl, the Drosophila ortholog of intellectual-disability-related ACSL4, inhibits synaptic growth by altered lipids. J Cell Sci 129:4034-4045 (2016). Read more (PubMed: 27656110) »

See all 14 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Jun 01 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Jun 01 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (Kidney)
Permeabilization
Yes - 0.1% troton x-100
Specification
Kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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Submitted May 18 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - 0.3% triton x-100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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Verified customer

Submitted May 18 2016

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Sample
African Green Monkey Cell (COS-7)
Specification
COS-7
Permeabilization
Yes - Saponin 0.05%
Fixative
Paraformaldehyde
Username

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Verified customer

Submitted Jan 31 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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