Overview

  • Product nameAnti-KDEL antibody [MAC 256]
    See all KDEL primary antibodies
  • Description
    Rat monoclonal [MAC 256] to KDEL
  • SpecificityThis monoclonal antibody reacts with the carboxy-terminal KDEL motif responsible for the endoplasmic reticulum (ER) localisation of several proteins. It is applicable as an ER marker in a wide variety of species (insects, mammals, plants) and also marks the ER-derived ‘ricinosome’ compartment in senescing plant tissue. It reacts strongly with protein
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with a wide range of species.
  • Immunogen

    Auxin binding protein (Maize) containing KDEL motif

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberMAC 256
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/300. PubMed: 20392701
WB Use at an assay dependent dilution.

Target

  • RelevanceThe sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins. 78 and 94 kDa glucose regulated proteins (GRP 78) and GRP 94 respectively and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.
  • Cellular localizationEndoplasmic reticulum
  • Alternative names
    • KDEL antibody
    • Lys Asp Glu Leu antibody

Anti-KDEL antibody [MAC 256] images

  • ab50601 at 1/300 dilution staining KDEL-ER Marker in Chinese Hamster Ovary cells by Immunocytochemistry/ Immunofluorescence. Cells were seeded in 24-well plates on glass coverslips coated with 25 μg/μl fibronectin. An ALexa Fluor labelled anti rat secondary was used at 5µg/ml.

References for Anti-KDEL antibody [MAC 256] (ab50601)

This product has been referenced in:
  • Cominelli A  et al. A unique C-terminal domain allows retention of matrix metalloproteinase-27 in the endoplasmic reticulum. Traffic 15:401-17 (2014). ICC/IF ; African Green Monkey . Read more (PubMed: 24548619) »
  • Pierrot N  et al. Amyloid precursor protein controls cholesterol turnover needed for neuronal activity. EMBO Mol Med 5:608-25 (2013). Rat . Read more (PubMed: 23554170) »

See all 12 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 30 µg
Specification Kidney
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Jun 01 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Brain)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 30 µg
Specification Brain
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Jun 01 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Monkey Cell (Kidney)
Permeabilization Yes - 0.1% troton x-100
Specification Kidney
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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Submitted May 18 2016

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Permeabilization Yes - 0.3% triton x-100
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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Submitted May 18 2016

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Sample African Green Monkey Cell (COS-7)
Specification COS-7
Permeabilization Yes - Saponin 0.05%
Fixative Paraformaldehyde
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Submitted Jan 31 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"