Overview

  • Product nameAnti-KDEL Receptor antibody [KR-10]See all KDEL Receptor primary antibodies ...
  • Description
    Mouse monoclonal [KR-10] to KDEL Receptor
  • Tested applicationsICC/IF, IHC-P, IHC-Fr, IP, IHC-FoFr, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Fruit fly (Drosophila melanogaster), Monkey
  • Immunogen

    Synthetic peptide derived from the sequence of bovine KDEL Receptor, conjugated to KLH

  • Positive control
    • HeLa cell lysate (heat shocked), 3T3 cell lysate (heat-shocked and unshocked), PC-12 cell lysate (heat shocked and unshocked) and CHO-K1 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab69659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
IHC-FoFr Use at an assay dependent dilution. PubMed: 20520821
WB 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
Flow Cyt Use 1µg for 106 cells.

Target

  • RelevanceRetention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi, or a pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually lys-asp-glu-leu (KDEL) in animal cells, and his-asp-glu-leu (HDEL) in S. cerevisiae. This process is mediated by a receptor that recognizes, and binds the tetrapeptide-containing protein, and returns it to the ER. In yeast, the sorting receptor encoded by a single gene, ERD2, which is a seven-transmembrane protein. Unlike yeast, several human homologs of the ERD2 gene, constituting the KDEL receptor gene family, have been described. The protein encoded by this gene was the first member of the family to be identified, and it encodes a protein structurally and functionally similar to the yeast ERD2 gene product.
  • Cellular localizationEndoplasmic reticulum
  • Database links
  • Alternative names
    • ER lumen protein retaining receptor 1 antibody
    • ERD 2 antibody
    • ERD2 antibody
    • ERD2.1 antibody
    • ERD21 antibody
    • HDEL antibody
    • KDEL (Lys Asp Glu Leu) endoplasmic reticulum protein retention receptor 1 antibody
    • KDEL (Lys-Asp-Glu-Leu) 72 kD endoplasmic reticulum protein retention receptor 1 antibody
    • KDEL endoplasmic reticulum protein retention receptor 1 antibody
    • KDEL R1 antibody
    • KDEL receptor 1 antibody
    • KDELR 1 antibody
    • KDELR1 antibody
    • PM 23 antibody
    • PM23 antibody
    • Putative MAPK activating protein PM23 antibody
    see all

Anti-KDEL Receptor antibody [KR-10] images

  • All lanes : Anti-KDEL Receptor antibody [KR-10] (ab69659) at 1/250 dilution

    Lane 1 : HeLa cell lysate (heat shocked)
    Lane 2 : 3T3 cell lysate
    Lane 3 : PC-12 cell lysate
    Lane 4 : CHO-K1 cell lysate
    Lane 5 : 3T3 cell lysate (heat shocked)
    Lane 6 : PC-12 cell lysate (heat shocked)

    Lysates/proteins at 20 µg per lane.


    Predicted band size : 25 kDa
    Observed band size : 25 kDa
  • ab69659 staining KDEL Receptor in Fruitfly epidermal cell tissue by Immunohistochemistry (PFA perfusion fixed frozen sections).
  • ab69659 staining the KDEL receptor in cultured human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X (0.5%) and blocked with 3% BSA for 1 hour at 24°C. Samples were incubated with primary antibody (1/100) for 1 hour at 24°C. An Alexa Fluor®488-conjugated Chicken anti-mouse polyclonal was used as the secondary antibody.

    See Abreview

  • Overlay histogram showing HepG2 cells stained with ab69659 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab69659, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-KDEL Receptor antibody [KR-10] (ab69659)

This product has been referenced in:
  • Kim S  et al. The novel endosomal membrane protein Ema interacts with the class C Vps-HOPS complex to promote endosomal maturation. J Cell Biol 188:717-34 (2010). Fruit fly (Drosophila melanogaster) . Read more (PubMed: 20194640) »
  • Norum M  et al. Trafficking through COPII stabilises cell polarity and drives secretion during Drosophila epidermal differentiation. PLoS One 5:e10802 (2010). IHC-FoFr ; Fruit fly (Drosophila melanogaster) . Read more (PubMed: 20520821) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - other (MEF)
Loading amount 30 µg
Specification MEF
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Verified customer

Submitted Nov 15 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (HELA)
Loading amount 30 µg
Specification HELA
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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Abcam user community

Verified customer

Submitted Nov 15 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cultured Cells (Mouse Embryonic Fibroblasts)
Specification Mouse Embryonic Fibroblasts
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Oct 15 2010

Application Immunocytochemistry
Sample Human Cultured Cells (HELA)
Specification HELA
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Oct 05 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"