Anti-KDELR2 antibody (ab74943)

Overview

  • Product nameAnti-KDELR2 antibody
    See all KDELR2 primary antibodies
  • Description
    Rabbit polyclonal to KDELR2
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal region of human KDELR2

  • Positive control
    • Extracts from HUVEC cells and COLO cells. A549 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab74943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionRequired for the retention of luminal endoplasmic reticulum proteins. Determines the specificity of the luminal ER protein retention system. Also required for normal vesicular traffic through the Golgi. This receptor recognizes K-D-E-L.
  • Sequence similaritiesBelongs to the ERD2 family.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • (Lys Asp Glu Leu) endoplasmic reticulum protein retention receptor 2 antibody
    • ELP 1 antibody
    • ELP-1 antibody
    • ELP1 antibody
    • ER lumen protein retaining receptor 2 antibody
    • ERD 2 like protein antibody
    • ERD2 like protein 1 antibody
    • ERD2-like protein 1 antibody
    • ERD2.2 antibody
    • ERD22_HUMAN antibody
    • FLJ45532 antibody
    • KDEL (Lys Asp Glu Leu) endoplasmic reticulum protein retention receptor 2 antibody
    • KDEL endoplasmic reticulum protein retention receptor 2 antibody
    • KDEL receptor 2 antibody
    • KDELR 2 antibody
    • kdelr2 antibody
    see all

Anti-KDELR2 antibody images

  • All lanes : Anti-KDELR2 antibody (ab74943) at 1/500 dilution

    Lane 1 : Extracts from HUVEC cells
    Lane 2 : Extracts from COLO cells
    Lane 3 : Extracts from HUVEC cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 24 kDa
    Observed band size : 24 kDa
    Additional bands at : 34 kDa,48 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of KDELR2 in A549 cells using ab74943, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.

References for Anti-KDELR2 antibody (ab74943)

ab74943 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (4-12% Bis Tris)
Sample Human Cell lysate - whole cell (lymphocytes)
Specification lymphocytes
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Feb 25 2014

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

As requested, I have obtained the testing protocols for this antibody and have copied these below. Ple...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"