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Ni-NTA purified truncated recombinant human LSD1 expressed in E. Coli strain BL21 (DE3).
Our Abpromise guarantee covers the use of ab31954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 19817750|
|IHC-P||1/500 - 1/2000.|
|IHC-Fr||1/500 - 1/2000.|
|WB||1/500 - 1/2000. Predicted molecular weight: 93 kDa.|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: KDM1 / LSD1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab31954 observed at 110 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab31954 was shown to recognize KDM1 / LSD1 when KDM1 / LSD1 knockout samples were used, along with additional cross-reactive bands. Wild-type and KDM1 / LSD1 knockout samples were subjected to SDS-PAGE. ab31954 and ab181602 (loading control to GAPDH) were diluted 1/250 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.