Anti-KDM1 / LSD1 antibody [1B2E5 + 1B2F2] (ab31954)

Overview

  • Product nameAnti-KDM1 / LSD1 antibody [1B2E5 + 1B2F2]
    See all KDM1 / LSD1 primary antibodies
  • Description
    Mouse monoclonal [1B2E5 + 1B2F2] to KDM1 / LSD1
  • Tested applicationsSuitable for: IHC-FoFr, IHC-P, IHC-Fr, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Ni-NTA purified truncated recombinant human LSD1 expressed in E. Coli strain BL21 (DE3).

  • Positive control
    • HeLa and Jurkat cell lysate for Western blot. Human kidnay and lung carcinoma tissue for Immunohistochemistry.

Properties

Applications

Our Abpromise guarantee covers the use of ab31954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 19817750
IHC-P 1/500 - 1/2000.
IHC-Fr 1/500 - 1/2000.
ELISA 1/10000.
WB 1/500 - 1/2000. Predicted molecular weight: 93 kDa.

Target

  • FunctionHistone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the flavin monoamine oxidase family.
    Contains 1 SWIRM domain.
  • DomainThe SWIRM domain may act as an anchor site for a histone tail.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Amine oxidase (flavin containing) domain 2 antibody
    • Amine oxidase, flavin containing, 2 antibody
    • AOF2 antibody
    • BHC110 antibody
    • BRAF35 HDAC complex protein BHC110 antibody
    • BRAF35-HDAC complex protein BHC110 antibody
    • BRAF35/HDAC complex, 110-kD subunit antibody
    • CPRF antibody
    • EC1 antibody
    • FAD binding protein BRAF35 HDAC complex, 110 kDa subunit antibody
    • Flavin-containing amine oxidase domain-containing protein 2 antibody
    • KDM 1 antibody
    • KDM1 antibody
    • Kdm1a antibody
    • KDM1A_HUMAN antibody
    • KIAA0601 antibody
    • LSD 1 antibody
    • LSD1 antibody
    • Lysine (K) specific demethylase 1 antibody
    • Lysine (K) specific demethylase 1A antibody
    • Lysine demethylase 1A antibody
    • Lysine specific histone demethylase 1 antibody
    • Lysine specific histone demethylase 1A antibody
    • Lysine-specific demethylase 1 antibody
    • Lysine-specific demethylase 1A antibody
    • Lysine-specific histone demethylase 1A antibody
    see all

Anti-KDM1 / LSD1 antibody [1B2E5 + 1B2F2] images



  • Predicted band size : 93 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: KDM1 / LSD1 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab31954 observed at 110 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab31954 was shown to recognize KDM1 / LSD1 when KDM1 / LSD1 knockout samples were used, along with additional cross-reactive bands. Wild-type and KDM1 / LSD1 knockout samples were subjected to SDS-PAGE. ab31954 and ab181602 (loading control to GAPDH) were diluted 1/250 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, showing nuclear localisation using LSD1 antibody (ab31954) dilution 1:500-1:2000 and an HRP-conjugated secondary antibody with DAB staining.
  • All lanes : Anti-KDM1 / LSD1 antibody [1B2E5 + 1B2F2] (ab31954)

    Lane 1 : Molecular weight marker
    Lane 2 : LSD1 recombinant protein (NB: truncated form)
    Lane 3 : HeLa cell lysate


    Predicted band size : 93 kDa
    Primary antibody dilution range 1:500-1:2000.
  • Immunohistochemical analysis of paraffin-embedded human kidney carcinoma tissue, showing nuclear localisation using LSD1 antibody (ab31954) dilution 1:500-1:2000 and an HRP-conjugated secondary antibody with DAB staining.

References for Anti-KDM1 / LSD1 antibody [1B2E5 + 1B2F2] (ab31954)

This product has been referenced in:
  • Hirono S  et al. Molecular markers associated with lymph node metastasis in pancreatic ductal adenocarcinoma by genome-wide expression profiling. Cancer Sci : (2009). IHC-FoFr ; Human . Read more (PubMed: 19817750) »

See 1 Publication for this product

Product Wall

I am very pleased to hear you would like to accept our offer and test ab31954 in mouse. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview formouse and include this code in the A...

Read More

Thanks for your reply.

I have requested the immunogen information used to produce ab90966. I will forward it to you as soon as I receive the details.

We typically only provide the discount testing program codes prior to purchase of ...

Read More

Thank you for your enquiry.

We do not have small sample sizes available. In lieu of this, we do have a testing program which you may be intersted in. I have described it below using ab90966 as an example. But the promotion could be applied to...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"