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Recombinant fragment (Human)
Our Abpromise guarantee covers the use of ab90966 in the following tested applications.
|Flow Cyt||Use 1-2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||1/1000. Predicted molecular weight: 93 kDa.|
|ELISA||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|ChIP||Use at an assay dependent concentration.|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: KDM1 / LSD1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab90966 observed at 110 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab90966 was shown to recognize KDM1 / LSD1 when KDM1 / LSD1 knockout samples were used, along with additional cross-reactive bands. Wild-type and KDM1 / LSD1 knockout samples were subjected to SDS-PAGE. ab90966 and ab181602 (loading control to GAPDH) were diluted to 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
ab90966 has not yet been referenced specifically in any publications.