Overview

  • Product nameAnti-KDM1 / LSD1 antibodySee all KDM1 / LSD1 primary antibodies ...
  • Description
    Rabbit polyclonal to KDM1 / LSD1
  • Tested applicationsICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Xenopus laevis
  • Immunogen

    Synthetic 16 amino acid peptide from near the centre of human KDM1/ LSD1.

  • Positive control
    • P815 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab62582 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 2 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 93 kDa).Can be blocked with KDM1 / LSD1 peptide (ab166968).

Target

  • FunctionHistone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the flavin monoamine oxidase family.
    Contains 1 SWIRM domain.
  • DomainThe SWIRM domain may act as an anchor site for a histone tail.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Amine oxidase (flavin containing) domain 2 antibody
    • Amine oxidase flavin containing domain 2 antibody
    • Amine oxidase flavin containing domain protein 2 antibody
    • AOF 2 antibody
    • AOF2 antibody
    • BHC110 antibody
    • BRAF35 HDAC complex protein BHC110 antibody
    • BRAF35-HDAC complex protein BHC110 antibody
    • FAD binding protein BRAF35 HDAC complex, 110 kDa subunit antibody
    • Flavin containing amine oxidase domain containing protein 2 antibody
    • Flavin-containing amine oxidase domain-containing protein 2 antibody
    • KDM 1 antibody
    • KDM1 antibody
    • Kdm1a antibody
    • KDM1A_HUMAN antibody
    • KIAA0601 antibody
    • LSD 1 antibody
    • LSD1 antibody
    • Lysine (K) specific demethylase 1 antibody
    • Lysine (K) specific demethylase 1A antibody
    • Lysine specific histone demethylase 1 antibody
    • Lysine specific histone demethylase 1A antibody
    • Lysine-specific histone demethylase 1A antibody
    see all

Anti-KDM1 / LSD1 antibody images

  • Lane 1 : Anti-KDM1 / LSD1 antibody (ab62582) at 1 µg/ml
    Lane 2 : Anti-KDM1 / LSD1 antibody (ab62582) at 2 µg/ml

    Lane 1 : P815 cell lysate
    Lane 2 : P815 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 93 kDa
    Observed band size : 105 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemical analysis of KDM1 / LSD1 expression in paraffin-embedded human small intestine tissue using ab62582 at 2 µg/ml.
  • Immunofluorescence of LSD1 in Human Small Intestine cells using ab62582 at 20 ug/ml.

References for Anti-KDM1 / LSD1 antibody (ab62582)

This product has been referenced in:
  • Krolewski RC  et al. Global expression profiling of globose basal cells and neurogenic progression within the olfactory epithelium. J Comp Neurol : (2012). WB ; Mouse . Read more (PubMed: 22847514) »

See 1 Publication for this product

Product Wall


The blocking peptide for ab62582 has been added as KDM1 / LSD1 peptide (ab166968).

http://www.abcam.com/index.html?datasheet=166968


As for the blocking peptides:

For ab129195: The blocking peptide is now available as ab166919.
http://www.abcam.com/index.html?datasheet=166919

For ab62582: This will be available soon and I will let you know when I have a ca...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Xenopus laevis Cell lysate - whole cell (egg extracts)
Loading amount 20 µg
Specification egg extracts
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
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Verified customer

Submitted Dec 21 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"