Anti-KDM1 / LSD1 antibody [1B2F2] (ab69535)

Overview

  • Product nameAnti-KDM1 / LSD1 antibody [1B2F2]
    See all KDM1 / LSD1 primary antibodies
  • Description
    Mouse monoclonal [1B2F2] to KDM1 / LSD1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant human LSD1 truncated protein

  • Positive control
    • HeLa cell extract NIH/3T3 cell extract
  • General notesConcentration: See product label

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab69535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 93 kDa).

Target

  • FunctionHistone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the flavin monoamine oxidase family.
    Contains 1 SWIRM domain.
  • DomainThe SWIRM domain may act as an anchor site for a histone tail.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Amine oxidase (flavin containing) domain 2 antibody
    • Amine oxidase, flavin containing, 2 antibody
    • AOF2 antibody
    • BHC110 antibody
    • BRAF35 HDAC complex protein BHC110 antibody
    • BRAF35-HDAC complex protein BHC110 antibody
    • BRAF35/HDAC complex, 110-kD subunit antibody
    • CPRF antibody
    • EC1 antibody
    • FAD binding protein BRAF35 HDAC complex, 110 kDa subunit antibody
    • Flavin-containing amine oxidase domain-containing protein 2 antibody
    • KDM 1 antibody
    • KDM1 antibody
    • Kdm1a antibody
    • KDM1A_HUMAN antibody
    • KIAA0601 antibody
    • LSD 1 antibody
    • LSD1 antibody
    • Lysine (K) specific demethylase 1 antibody
    • Lysine (K) specific demethylase 1A antibody
    • Lysine demethylase 1A antibody
    • Lysine specific histone demethylase 1 antibody
    • Lysine specific histone demethylase 1A antibody
    • Lysine-specific demethylase 1 antibody
    • Lysine-specific demethylase 1A antibody
    • Lysine-specific histone demethylase 1A antibody
    see all

Anti-KDM1 / LSD1 antibody [1B2F2] images



  • Predicted band size : 93 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: KDM1 / LSD1 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab69535 observed at 110 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab69535 was shown to specifically react with KDM1 / LSD1 when KDM1 / LSD1 knockout samples were used. Wild-type and KDM1 / LSD1 knockout samples were subjected to SDS-PAGE. ab69535 and ab181602 (loading control to GAPDH) were diluted to 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-KDM1 / LSD1 antibody [1B2F2] (ab69535) at 1 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : HeLa cell extract
    Lane 3 : NIH/3T3 cell extract

    Developed using the ECL technique

    Predicted band size : 93 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 112 kDa. We are unsure as to the identity of these extra bands.

References for Anti-KDM1 / LSD1 antibody [1B2F2] (ab69535)

ab69535 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab69535.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"