KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric) (ab113459)

Overview

  • Product nameKDM1/LSD1 Activity Quantification Assay Kit (Colorimetric)See all KDM1 / LSD1 kits ...
  • Assay time
    3h 00m
  • Species reactivity
    Reacts with: Mouse, Human, Plants
    Predicted to work with: all Mammals, Other
  • Product overview

    KMD1/LSD1 (Lysine Specific Histone Demethylase 1) is a histone demethylase that only acts on mono- or di-methylated lysines. KDM1/LSD1 acts as a co-repressor by mediating demethylation of H3(meK4), a specific tag for epigenetic transcriptional activation.

     

    KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric) (ab113459) is designed for the easy and fast colorimetric measurement of KDM1/LSD1 activity or inhibition. The antibody-based, immunospecific method directly detects KDM1/LSD1-converted demethylated products, rather than by-products, in a 96 stripwell microplate format.

  • Tested applicationsFunctional Studiesmore details

Properties

  • RelevanceHistone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2)H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor(ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development.
  • Alternative names
    • Amine oxidase (flavin containing) domain 2
    • AOF2
    • BHC110
    • BRAF35-HDAC complex protein BHC110
    • FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit
    • Flavin-containing amine oxidase domain-containing protein 2
    • KDM1
    • KIAA0601
    • LSD1
    • Lysine (K) specific demethylase 1
    • Lysine (K) specific demethylase 1A
    • Lysine-specific histone demethylase 1
    • Lysine-specific histone demethylase 1A
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab113459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric) images

  • Demonstration of inhibitory effects of an KDM1/LSD1 inhibitor detected by ab113459. [LSD1 concentration: 200 ng/well]
  • Demonstration of high sensitivity of KDM1 / LSD1 activity assay achieved by using recombinant KDM1 / LSD1 with ab113459.

Protocols

References for KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric) (ab113459)

ab113459 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.
The kit ab113472 is designed to detect DNA demethylase activity and will detect the this activity using a unique methylated DNA substrate. The detection is then colorimetric and can be measured by optical density measu...

Read More

Thank you for contacting Abcam. For ab113459 and ab113460, both kits directly detects KDM1/LSD1-converted demethylated products, rather than by-products, in a 96 stripwell microplate format. The main difference between the 2 are that ab113459, has a co...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"