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KMD1/LSD1 (Lysine Specific Histone Demethylase 1) is a histone demethylase that only acts on mono- or di-methylated lysines. KDM1/LSD1 acts as a co-repressor by mediating demethylation of H3 (meK4), a specific tag for epigenetic transcriptional activation.
KDM1/LSD1 Activity Quantification Assay Kit (Fluorometric) (ab113460) is designed for the easy and fast fluorometric measurement of KDM1/LSD1 activity or inhibition. The antibody-based, immunospecific method directly detects KDM1/LSD1-converted demethylated products, rather than by-products, in a 96 stripwell microplate format.
|Components||48 tests||96 tests|
|10X Wash Buffer||1 x 14ml||1 x 28ml|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|Adhesive Covering Film||1 unit||1 unit|
|Capture Antibody, 1000 µg/mL||1 x 5µl||1 x 10µl|
|Detection Antibody, 400 µg/mL||1 x 6µl||1 x 12µl|
|Fluoro Developer||1 x 10µl||1 x 20µl|
|Fluoro Diluter||1 x 4ml||1 x 8ml|
|Fluoro Enhancer||1 x 10µl||1 x 20µl|
|LSD1 Assay Buffer||1 x 4ml||1 x 8ml|
|LSD1 Assay Standard, 50 µg/mL||1 x 10µl||1 x 20µl|
|LSD1 Inhibitor Tranylcypromine, 1 mM||1 x 20µl||1 x 40µl|
|LSD1 Substrate, 50 µg/ml||1 x 60µl||1 x 120µl|
Our Abpromise guarantee covers the use of ab113460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab113460 has not yet been referenced specifically in any publications.