Human Predicted to work with:
Mouse, Rat, Rabbit, Cow, Dog, Pig, Macaque monkey, Gorilla
Synthetic peptide corresponding to Human KDM2B aa 1-100 conjugated to keyhole limpet haemocyanin. Database link: Q8NHM5 (Peptide available as ab167447)
This antibody gave a positive signal in both HeLa whole cell lysate and HeLa Nuclear lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 165 kDa (predicted molecular weight: 152 kDa).
Histone demethylase that demethylates 'Lys-4' and 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' and dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. Preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which inhibits cell growth and proliferation. May also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
The predicted molecular weight of KDM2B is 152 kDa (SwissProt), however we expect to observe a banding pattern around 165 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Jiang Y et al. Local generation of fumarate promotes DNA repair through inhibition of histone H3 demethylation. Nat Cell Biol17:1158-68 (2015).
Read more (PubMed: 26237645) »
Lu S et al. The transcription factor c-Fos coordinates with histone lysine-specific demethylase 2A to activate the expression of cyclooxygenase-2. Oncotarget6:34704-17 (2015).
Read more (PubMed: 26430963) »