Anti-KDM2B antibody (ab5199)
Key features and details
- Goat polyclonal to KDM2B
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KDM2B antibody
See all KDM2B primary antibodies -
Description
Goat polyclonal to KDM2B -
Host species
Goat -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Dictyostelium discoideum -
Immunogen
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5199 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 1 - 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use a concentration of 1 - 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Histone demethylase that demethylates 'Lys-4' and 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' and dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. Preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which inhibits cell growth and proliferation. May also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. -
Sequence similarities
Belongs to the JHDM1 histone demethylase family.
Contains 1 CXXC-type zinc finger.
Contains 1 F-box domain.
Contains 1 JmjC domain.
Contains 7 LRR (leucine-rich) repeats.
Contains 1 PHD-type zinc finger. -
Domain
The JmjC domain mediates demethylation activity (By similarity). It is also required for repression of ribosomal RNA genes. -
Cellular localization
Nucleus > nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 84678 Human
- Omim: 609078 Human
- SwissProt: Q8NHM5 Human
- Unigene: 524800 Human
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Alternative names
- [Histone-H3]-lysine-36 demethylase 1B antibody
- CXXC-type zinc finger protein 2 antibody
- CXXC2 antibody
see all
Datasheets and documents
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Datasheet download
References (8)
ab5199 has been referenced in 8 publications.
- Saha T et al. Intestinal TMEM16A control luminal chloride secretion in a NHERF1 dependent manner. Biochem Biophys Rep 25:100912 (2021). PubMed: 33537462
- Vargas-Ayala RC et al. Interplay between the Epigenetic Enzyme Lysine (K)-Specific Demethylase 2B and Epstein-Barr Virus Infection. J Virol 93:N/A (2019). PubMed: 30996097
- Yan M et al. miR-146b promotes cell proliferation and increases chemosensitivity, but attenuates cell migration and invasion via FBXL10 in ovarian cancer. Cell Death Dis 9:1123 (2018). PubMed: 30409964
- Zhang DD et al. Vildagliptin, a DPP4 inhibitor, alleviates diabetes-associated cognitive deficits by decreasing the levels of apoptosis-related proteins in the rat hippocampus. Exp Ther Med 15:5100-5106 (2018). PubMed: 29805536
- He P et al. The NHERF1 PDZ1 domain and IRBIT interact and mediate the activation of Na+/H+ exchanger 3 by ANG II. Am J Physiol Renal Physiol 311:F343-51 (2016). PubMed: 27279487
- He P et al. Restoration of Na+/H+ exchanger NHE3-containing macrocomplexes ameliorates diabetes-associated fluid loss. J Clin Invest 125:3519-31 (2015). PubMed: 26258413
- Tzatsos A et al. Lysine-specific Demethylase 2B (KDM2B)-let-7-Enhancer of Zester Homolog 2 (EZH2) Pathway Regulates Cell Cycle Progression and Senescence in Primary Cells. J Biol Chem 286:33061-9 (2011). WB ; Mouse . PubMed: 21757686
- Ge R et al. F-box protein 10, an NF-?B-dependent anti-apoptotic protein, regulates TRAIL-induced apoptosis through modulating c-Fos/c-FLIP pathway. Cell Death Differ 18:1184-95 (2011). PubMed: 21252908